Anti-SLC18B1 (VPAT) Antibody | Alomone Labs AMT-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC18B1 (VPAT) Antibody

catalog :

AMT-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1-2. Anti-SLC18B1 (VPAT) Antibody (#AMT-008), (1:200). 3-4. Anti-SLC18B1 (VPAT) Antibody, preincubated with SLC18B1 (VPAT) Blocking Peptide (BLP-MT008).

image 2 :

Expression of SLC18B1 in mouse striatum. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC18B1 (VPAT) Antibody (#AMT-008), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SLC18B1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with SLC18B1 (VPAT) Blocking Peptide (BLP-MT008), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AMT-008

SKU :

AMT-008-CF_0.2 ml

Product Name :

Anti-SLC18B1 (VPAT) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

D3Z5L6

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT008

Homology :

Rat - 13 out of 14 amino acid residues identical, Human - 10 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ESDPGKQSFWKLVT, corresponding to amino acid residues 222 - 235 of mouse SLC18B1

Product Page - Scientific background :

The vesicular amine transporter family (VAT, SLC18) is a group of integral proteins located at the secretory vesicles of neuronal and endocrine cells. SLC18 transporters are responsible for the transition of neurotransmitters such as serotonin, adrenaline, dopamine, acetylcholine and histamine into secretory vesicles1,4. Solute carrier 18B1 (SLC18B1) is the fourth and last identified member of the SLC18 family and is conserved across all kingdom of life. In contrast to the other SLC18 proteins that can recognized only monoamines as a substrate, SLC18B1 can recognize diamines and triamines such as spermidine (Spd) and spermine (Spm). The transporter carries out this function using a gradient of H+ created by vacuolar H+-ATPase activity2. SLC18B1 has a predicted membrane topology of 12 transmembrane domains (TMDs) and two phosphorylation sites. SLC18B1 mRNA was found to be expressed in both human and mice in the lung, placenta, adrenal gland, liver and brain. It was found that SLC18B1 knock out mice has impaired short and long term memory. In addition, SLC18B1 KO eliminate the secretion of histamine from mice mast cells and therefore play a role in the immune response3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Vesicular Polyamine Transporter, Solute Carrier Family 18 Member B1, MFS-Type Transporter SLC18B1

Short Description :

A Rabbit Polyclonal antibody to SLC18B1 (VPAT)

Long Description :

Anti-SLC18B1 (VPAT) Antibody (#AMT-008) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC18B1 from rat, mouse and human samples.

Negative Control :

BLP-MT008

Positive Control :

Synonyms :

Vesicular Polyamine Transporter, Solute Carrier Family 18 Member B1, MFS-Type Transporter SLC18B1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC18B1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage