Anti-VMAT1 Antibody | Alomone Labs AMT-007 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-VMAT1 Antibody

catalog :

AMT-007

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Honke N, Lowin T, Opgenoorth B, Shaabani N, Lautwein A, Teijaro J, et al. Endogenously produced catecholamines improve the regulatory function of TLR9-activated B cells. PLoS Biol. 2022;20:e3001513 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-VMAT1 Antibody (#AMT-007), (1:200).3,4. Anti-VMAT1 Antibody, preincubated with VMAT1 Blocking Peptide (#BLP-MT007).

product information

CAT :

AMT-007

SKU :

AMT-007-CF_0.2 ml

Product Name :

Anti-VMAT1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01818

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT007

Homology :

Mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st luminal loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFKDSNSSLHRGPS, corresponding to amino acid residues 53-66 of rat VMAT1

Product Page - Scientific background :

Vesicular monoamine transporters (VMAT) are transmembrane proteins located on membranes of monoaminergic neurons and are responsible for the uptake of cytosolic monoamines into synaptic vesicles. They store neurotransmitter molecules in synaptic vesicles for additional cycles of release. They are acidic glycoproteins encoded by the SLC18A1 gene and transport neurotransmitters including dopamine, serotonin, adrenaline, noradrenaline, histamine and norepinephrine1,2. Its structure is composed of 12 transmembrane domains, the C and N-terminus are located in the cytosolic side of the vesicle. The glycosylation sites are located in the vesicular matrix on a loop between transmembrane domain I and II1.VMAT have an important role in sorting and releasing of neurotransmitters and in the adjustment of neuronal and endocrine informational output. The transport into the storage organelle is dependent on a vacuolar ATPase-generated proton gradient in exchange for protons.Two monoamine transporters have been identified in rats and in human: VMAT1 and VMAT2. In humans, VMAT1 is preferentially expressed in large dense core vesicles of various neuroendocrine cells, including chromaffin and enterochromaffin cells.Several studies have suggested that VMAT1 has a strong connection to schizophrenia, bipolar disorder, and other anxiety related personality disorders, partially due to the fact that VMAT1 maps to chromosome 8p21.3, a locus with strong evidence for linkage with schizophrenia1,3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Vesicular monoamine transporter 1, SLC18A1, CGAT, VAT1

Short Description :

A Rabbit Polyclonal Antibody to Vesicular Monoamine Transporter 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat vesicular monoamine transporter 1. Anti-VMAT1 Antibody (#AMT-007) can be used in western blot analysis. It has been designed to recognize VMAT1 from rat and mouse samples. The antibody is not recommended to be used on human samples.

Negative Control :

BLP-MT007

Positive Control :

Synonyms :

Vesicular monoamine transporter 1, SLC18A1, CGAT, VAT1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC18A2

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage