Anti-VMAT2 Antibody | Alomone Labs AMT-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-VMAT2 Antibody

catalog :

AMT-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Honke N, Lowin T, Opgenoorth B, Shaabani N, Lautwein A, Teijaro J, et al. Endogenously produced catecholamines improve the regulatory function of TLR9-activated B cells. PLoS Biol. 2022;20:e3001513 pubmed publisher
Kramer D, Aisenberg E, Kosillo P, Friedmann D, Stafford D, Lee A, et al. Generation of a DAT-P2A-Flpo mouse line for intersectional genetic targeting of dopamine neuron subpopulations. Cell Rep. 2021;35:109123 pubmed publisher
Zhao J, Munanairi A, Liu X, Zhang J, Hu L, Hu M, et al. PAR2 Mediates Itch via TRPV3 Signaling in Keratinocytes. J Invest Dermatol. 2020;140:1524-1532 pubmed publisher
Peixoto Neves D, Soni H, Adebiyi A. Oxidant-induced increase in norepinephrine secretion from PC12 cells is dependent on TRPM8 channel-mediated intracellular calcium elevation. Biochem Biophys Res Commun. 2018;506:709-715 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain stem (lanes 3 and 6) lysates: - 1-3. Anti-VMAT2 Antibody (#AMT-006), (1:400).4-6. Anti-VMAT2 Antibody, preincubated with VMAT2 Blocking Peptide (#BLP-MT006).

image 2 :

Expression of VMAT2 in rat substantia nigra dopaminergic neurons - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-VMAT2 Antibody (#AMT-006), (1:1000). A. VMAT2 staining (red). B. Tyrosine hydroxylase staining (green), a marker of dopaminergic neurons. C. Merger of A and B demonstrates VMAT2 expression in several dopaminergic neurons (arrows). Cell nuclei were stained with DAPI (blue).

image 3 :

Immuno-colocalization of VMAT2 and TRPC3 in rat brain - Immunohistochemical staining of perfusion-fixed frozen rat substantia nigra sections using Anti-VMAT2-ATTO-488 Antibody(#AMT-006-AG) (1:60) andAnti-TRPC3-ATTO-594 Antibody(#ACC-016-AR) (1:60). A. VMAT2 staining (green). B. The same section stained for TRPC3 (red). C. Merged image demonstrates the ubiquitous colocalization of VMAT2 and TRPC3 in cells of the substantia nigra pars compacta. Arrows point at examples of co-expression.

product information

CAT :

AMT-006

SKU :

AMT-006-CF_0.2 ml

Product Name :

Anti-VMAT2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01827

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT006

Homology :

Rat, mouse, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st luminal loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KHEKNSTEIQT(A)R, corresponding to amino acid residues 52-64 of rat VMAT2

Product Page - Scientific background :

Vesicular monoamine transporters (VMATs) are essential for proper monoaminergic neurotransmission, which requires the sequestration of the transmitter into synaptic vesicles by VMAT for subsequent Ca2+-stimulated exocytotic release. VMATs contain 12 transmembrane spanning domains, with cytosolic C- and N-terminals and large glycosylated intravesicular loops. In mammals, there are two VMAT isoforms: VMAT1 (SLC18A1) and VMAT2 (SLC18A2)1.VMATs primarily transport monoamines (dopamine, serotonin, norepinephrine and histamine), but also sequester toxicants into vesicles, shunting them away from cytosolic sites of action. In the central nervous system, VMAT2 is the only transporter that moves cytoplasmic dopamine (DA) into synaptic vesicles for storage and subsequent exocytotic release2.VMAT2 is expressed in monoaminergic neurons of the central nervous system (CNS) and sympathetic postganglionic neurons. VMAT2 is widely expressed during embryonic development3. Homozygous knock-out of VMAT2 in mice is lethal and heterozygous knock-out of VMAT2 in mice shows a clear gene dosage effect, with half the amount of dopamine, norepinephrine, and serotonin content in brain4. In addition VMAT2 plays a role in neuroprotection and molecules that interact with VMAT2 may have value as treatments for diseases such as Parkinson's disease5.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2

Short Description :

A Rabbit Polyclonal Antibody to Vesicular Monoamine Transporter 2

Long Description :

Anti-VMAT2 Antibody (#AMT-006) is a highly specific antibody directed against an epitope of the rat vesicular monoamine transporter 2. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize VMAT2 from human, rat and mouse samples.

Negative Control :

BLP-MT006

Positive Control :

Synonyms :

Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC18A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage