Anti-VMAT2-ATTO Fluor-488 Antibody | Alomone Labs AMT-006-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-VMAT2-ATTO Fluor-488 Antibody

catalog :

AMT-006-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Multiplex staining of VMAT2 and TRPC3 in rat brain - Immunohistochemical staining of perfusion-fixed frozen rat substantia nigra sections using Anti-VMAT2-ATTO Fluor-488 Antibody (#AMT-006-AG), (1:60) and Anti-TRPC3-ATTO Fluor-594 Antibody (#ACC-016-AR), (1:60). A. VMAT2 staining (green). B. The same section stained for TRPC3 (red). C. Merged image demonstrates the ubiquitous colocalization of VMAT2 and TRPC3 in cells of the substantia nigra pars compacta. Arrows point at examples of co-expression.

image 2 :

Alomone Labs GYKI 53655 hydrochloride inhibits GluA1 channels expressed inXenopusoocytes. - Representative time course of GluA1 current activated by a continuous application (top dotted line) of 1 M(S)-AMPA(#A-267) and reversibly inhibited by 50 MGYKI 53655 hydrochloride(#G-220) as indicated (bar) at a holding potential of -80 mV.

product information

CAT :

AMT-006-AG

SKU :

AMT-006-AG_50 mcl

Product Name :

Anti-VMAT2-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01827

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT006

Homology :

Rat, mouse, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

1st luminal loop

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KHEKNSTEIQT(A)R, corresponding to amino acid residues 52-64 of rat VMAT2

Product Page - Scientific background :

Vesicular monoamine transporters (VMATs) are essential for proper monoaminergic neurotransmission, which requires the sequestration of the transmitter into synaptic vesicles by VMAT for subsequent Ca2+-stimulated exocytotic release. VMATs contain 12 transmembrane spanning domains, with cytosolic C- and N-terminals and large glycosylated intravesicular loops. In mammals, there are two VMAT isoforms: VMAT1 (SLC18A1) and VMAT2 (SLC18A2)1.VMATs primarily transport monoamines (dopamine, serotonin, norepinephrine and histamine), but also sequester toxicants into vesicles, shunting them away from cytosolic sites of action. In the central nervous system, VMAT2 is the only transporter that moves cytoplasmic dopamine (DA) into synaptic vesicles for storage and subsequent exocytotic release2.VMAT2 is expressed in monoaminergic neurons of the central nervous system (CNS) and sympathetic postganglionic neurons. VMAT2 is widely expressed during embryonic development3. Homozygous knock-out of VMAT2 in mice is lethal and heterozygous knock-out of VMAT2 in mice shows a clear gene dosage effect, with half the amount of dopamine, norepinephrine, and serotonin content in brain4. In addition VMAT2 plays a role in neuroprotection and molecules that interact with VMAT2 may have value as treatments for diseases such as Parkinson's disease5.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2

Short Description :

A Rabbit Polyclonal Antibody to Vesicular Monoamine Transporter 2 Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-VMAT2 Antibody (#AMT-006) is a highly specific antibody directed against an epitope of the rat vesicular monoamine transporter 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize VMAT2 from human, rat and mouse samples. Anti-VMAT2-ATTO Fluor-488 Antibody (#AMT-006-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-VMAT2-ATTO Fluor-488 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC18A2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AMT-006), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage