Anti-Dopamine Transporter (DAT) (extracellular) Antibody | Alomone Labs AMT-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Dopamine Transporter (DAT) (extracellular) Antibody

catalog :

AMT-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Kikuta S, Iguchi Y, Kakizaki T, Kobayashi K, Yanagawa Y, Takada M, et al. Store-Operated Calcium Channels Are Involved in Spontaneous Slow Calcium Oscillations in Striatal Neurons. Front Cell Neurosci. 2019;13:547 pubmed publisher
Mashima K, Takahashi S, Minami K, Izawa Y, Abe T, Tsukada N, et al. Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway. ASN Neuro. 2018;10:1759091418775562 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 4) and mouse (lanes 2 and 5) brain membranes and rat pheochromocytoma PC12 (lanes 3 and 6) cell lysates: - 1-3. Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003), (1:200).4-6. Anti-Dopamine Transporter (DAT) (extracellular) Antibody, preincubated with Dopamine Transporter/DAT (extracellular) Blocking Peptide (#BLP-MT003).

image 2 :

Peptide (C)DAHASNSSDGLGLND corresponding to amino acids residues 191-205 of rat DAT (Accession P23977). 2nd extracellular loop.

image 3 :

Expression of DAT in rat PC12 cells - Cell surface detection of DAT in live intact rat pheochromocytoma PC12 cells. A. Cells stained with Anti-Dopamine Transporter (DAT) (extracellular)Antibody (#AMT-003) (1:100) followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Live view of the cells.

product information

CAT :

AMT-003

SKU :

AMT-003-CF_0.2 ml

Product Name :

Anti-Dopamine Transporter (DAT) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23977

Applications :

IC IFC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT003

Homology :

Mouse - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT

Product Page - Scientific background :

The Na+/Cl- transporter family SLC6, includes DAT, a monoamine transporter, important for regulating extracellular levels of dopamine. It does so by taking up dopamine from the synaptic cleft via the co-transport of Na+ and Cl- down their electrochemical gradients1. The removal of dopamine by DAT remains the most important means to control the extracellular lifetime of the neurotransmitter and notably, the ending of dopaminergic neurotransmission2. Transporters for serotonin, norepinephrine, GABA and glycine also belong to this family3.These receptors have 12 transmembrane spanning domains and intracellular N- and C-termini. DAT is also subject to post translational modifications such as phosphorylation, important for its regulation1. It also possesses a large extracellular domain which undergoes N-glycosylation, important for the proper targeting of the transporter to the plasma membrane1. DAT is expressed in dopaminergic cell bodies and terminals and can therefore serve as a marker for these neurons4. DAT is also expressed in the retina, gastrointestinal tract, lung, kidney, pancreas and lymphocytes2.DAT plays an important role in movement as well as reward, learning and memory5. Its malfunction, which leads to dopaminergic dysregulation, has been associated with ADHD, schizophrenia, as well as Parkinson's disease2. DAT is the main target for cocaine, amphetamine and methamphetamine psychostimulants, which mainly increase locomotor activities2.

Applications may also work in :

IC IFC LCI WB

Supplier :

Alomone Labs

Target :

Sodium-dependent dopamine transporter, DA transporter, SLC6A3

Short Description :

A Rabbit Polyclonal Antibody to Dopamine Transporter (DAT)

Long Description :

Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize DAT from mouse and rat samples. The antibody is unlikely to recognize DAT from human samples.

Negative Control :

BLP-MT003

Positive Control :

Synonyms :

Sodium-dependent dopamine transporter, DA transporter, SLC6A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A3

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage