Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody | Alomone Labs AMT-003-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody

catalog :

AMT-003-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of DAT in mouse J774 macrophages: Cell surface detection of DAT in mouse J774 macrophages: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F), (5 µg).

product information

CAT :

AMT-003-F

SKU :

AMT-003-F_50 mcl

Product Name :

Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23977

Applications :

FC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT003

Homology :

Mouse - 14/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT

Product Page - Scientific background :

The Na+/Cl- transporter family SLC6, includes DAT, a monoamine transporter, important for regulating extracellular levels of dopamine. It does so by taking up dopamine from the synaptic cleft via the co-transport of Na+ and Cl- down their electrochemical gradients1. The removal of dopamine by DAT remains the most important means to control the extracellular lifetime of the neurotransmitter and notably, the ending of dopaminergic neurotransmission2. Transporters for serotonin, norepinephrine, GABA and glycine also belong to this family3.These receptors have 12 transmembrane spanning domains and intracellular N- and C-termini. DAT is also subject to post translational modifications such as phosphorylation, important for its regulation1. It also possesses a large extracellular domain which undergoes N-glycosylation, important for the proper targeting of the transporter to the plasma membrane1. DAT is expressed in dopaminergic cell bodies and terminals and can therefore serve as a marker for these neurons4. DAT is also expressed in the retina, gastrointestinal tract, lung, kidney, pancreas and lymphocytes2.DAT plays an important role in movement as well as reward, learning and memory5. Its malfunction, which leads to dopaminergic dysregulation, has been associated with ADHD, schizophrenia, as well as Parkinson's disease2. DAT is the main target for cocaine, amphetamine and methamphetamine psychostimulants, which mainly increase locomotor activities2.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Sodium-dependent dopamine transporter, DA transporter, Solute carrier family 6 member 3, SLC6A3

Short Description :

A Rabbit Polyclonal Antibody to Dopamine Transporter (DAT) Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize DAT from mouse and rat samples. The antibody is unlikely to recognize DAT from human samples. Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Sodium-dependent dopamine transporter, DA transporter, Solute carrier family 6 member 3, SLC6A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A3

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AMT-003), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage