Anti-Noradrenaline Transporter (NET) (extracellular) Antibody | Alomone Labs AMT-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Noradrenaline Transporter (NET) (extracellular) Antibody

catalog :

AMT-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Rodrigues Amorim D, Iglesias Martínez Almeida M, Rivera Baltanas T, Fernández Palleiro P, Freiría Martínez L, Rodríguez Jamardo C, et al. The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia. Int J Mol Sci. 2021;22: pubmed publisher
Mashima K, Takahashi S, Minami K, Izawa Y, Abe T, Tsukada N, et al. Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway. ASN Neuro. 2018;10:1759091418775562 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002), (1:400).3,4. Anti-Noradrenaline Transporter (NET) (extracellular) Antibody, preincubated with Noradrenaline Transporter/NET (extracellular) Blocking Peptide (#BLP-MT002).

image 2 :

Expression of Noradrenaline transporter (NET) in rat hippocampal dentate gyrus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002), (1:200). A. NET (green) is visualized in axonal processes (arrows) in the hilus. B. The relation to layers was explored using mouse anti-GAP43 (red). There are less axonal processes in the outer molecular layer (asterisk) compared to the inner molecular layer (arrows). C. Overlay of A and B shows co-localization of NET and GAP43 in the outer molecular layer.

image 3 :

Expression ofNoradrenaline transporter (NET) in rat hippocampal dentate gyrus - Immunohistochemical staining of immersion-fixed free floating rat brain frozen sections usingAnti-Noradrenaline Transporter (NET) (extracellular) Antibody(#AMT-002) (1:200). A. NET (green) is visualized in axonal processes (arrows) in the hilus. B. The relation to layers was explored using mouse anti-GAP43 (red). There are less axonal processes in the outer molecular layer (asterisk) compared to the inner molecular layer (arrows). C. Overlay of A and B shows co-localization of NET and GAP43 in the outer molecular layer.

product information

CAT :

AMT-002

SKU :

AMT-002-CF_0.2 ml

Product Name :

Anti-Noradrenaline Transporter (NET) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O55192

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MT002

Homology :

Rat - identical; human - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KLLNASVLGDHTKYSK, corresponding to amino acid residues 189-204 of mouse NET

Product Page - Scientific background :

Many physiological, endocrine and behavioral functions are determined and regulated by monoamine signaling1,2. Many brain disorders such as depression, drug abuse, schizophrenia, attention deficit hyperactivity disorder (ADHD) are caused by the malfunction of monoaminergic transmission1-3. The intensity of monoaminergic signaling is determined by the availability of the monoamine, which is in turn determined in part by its uptake from the extracellular milieu via monoamine transporters. These transporters include DAT, SERT, and NET, responsible for uptaking dopamine, serotonin and noradrenaline respectively, and recycling them back for release3-5.While the activity of each transporter is faithful to its neurotransmitter, NET has been shown to clear dopamine in DAT deprived or low DAT regions such as the brain cortex6-8.DAT, SERT and NET are members of the Na+/Cl- dependent membrane transporter family which also includes other members. These transporters consist of 12 transmembrane domains and intracellular N- and C-termini. NET also has a significant extracellular loop between transmembrane regions three and four, which contains various glycosylation sites9. Like its counterparts, NET's intracellular N- and C-terminal domains are also subject to phosphorylation and protein-protein interactions important for modulating its activity and localization. In addition alternative splicing has also been shown to regulate NET's expression and function10.NET is specifically expressed on noradrenaline nerve terminals, and is also expressed in the periphery, such as adrenal glands and placenta. NET malfunction is largely associated with attention and mood, as well as various cardiovascular disorders. NET is also a target for psychostimulants such as cocaine and amphetamines which disrupt its function, thereby causing an increase of noradrenaline in synaptic clefts3,9.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Sodium-dependent noradrenaline transporter, Norepinephrine transporter, SLC6A2

Short Description :

A Rabbit Polyclonal Antibody to Noradrenaline Transporter (NET)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse noradrenaline (norepinephrine) transporter. Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002) can be used in western blot immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NET from human, mouse and rat samples.

Negative Control :

BLP-MT002

Positive Control :

Synonyms :

Sodium-dependent noradrenaline transporter, Norepinephrine transporter, SLC6A2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC6A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage