antibody

Anti-Human MRGPRX2 (extracellular) Antibody

AMR-072

polyclonal

domestic rabbit

nonconjugated

NA

AMR-072-CF_0.2 ml

Anti-Human MRGPRX2 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q96LB1

IFC LCI WB

Human

Rabbit

BLP-MR072

Human only No cross reactivity with mouse or rat related proteins

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

(C)TPAWGTESTTVNGND, corresponding to amino acid residues 5 - 19 of human MRGPRX2

MRGPRX2, also known as Mas-related G protein-coupled receptor member X2, is a member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily and the Mas-related subfamily branch of the rhodopsin-like class A GPCRs or Mas-related G protein-coupled receptors (Mrgprs).1,2Mrgprs first described 25 years ago, comprise more than 50 receptors in humans and rodents, most of which are still considered orphan receptors, that is, receptors with no identified endogenous or synthetic ligands.1.2The MRGPRX subfamily consists of four proteins (MRGPRX1 to MRGPRX4). The four proteins have sequence similarity with the MrgA Mas-related subfamily, however, the MRGPRX genes are only present in primates with no true orthologues in mice or rats. 1,2MRGPRX2 highest expression is observed in mast cells, although it has also been identified in other peripheral blood cells, including basophils and eosinophils, and at low levels in other tissues, such as the gastrointestinal tract and the central nervous system.1.2The expression and function of MRGPRX2 in mast cells garnered considerable interest, as these cells play a crucial role in the immune system and are involved in allergic reactions and inflammation. Indeed, MRGPRX2 activation in mast cells, triggers a signaling pathway that leads to the release of various inflammatory mediators, such as histamine, cytokines, and chemokines. This release of mediators contributes to the initiation and amplification of inflammatory responses. 3-5The mast cell-mediated classical allergic response, involves crosslinking of high affinity immunoglobulin E (IgE) receptor (FcεRI) on mast cells with allergen, which then release a wide range of pre-stored inflammatory mediators (histamine, serotonin, protease), followed by de novo synthesis of mediators (prostaglandins and leukotrienes) and a variety of cytokines such as interleukin (IL)-4, IL-5, IL-9, and IL-13. 3-5MRGPRX2 has been linked to pseudo allergic reactions, which mimic true allergic reactions but do not involve IgE antibodies. Pseudo allergic reactions can be triggered by substances that directly activate MRGPRX2 on mast cells, leading to the release of histamine and other inflammatory mediators.3-5In addition, expression of MRGPRX2 in sensory neurons, may contribute to the sensation of itch and pain, thus highlighting its role in neurogenic inflammation beyond classical allergic responses.3-5

IFC LCI WB

Alomone Labs

Mas-related G-protein coupled receptor member X2, MRGX2

A Rabbit Polyclonal antibody to Human MRGPRX2 (extracellular)

Anti-Human MRGPRX2 (extracellular) Antibody (#AMR-072) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize the human MRGPRX2 protein. It won’t recognize any mouse or rat related proteins.

BLP-MR072

NA

Mas-related G-protein coupled receptor member X2, MRGX2

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

MRGPRX2

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200