Anti-MRGPRD (GPCR TGR7) Antibody | Alomone Labs AMR-061 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MRGPRD (GPCR TGR7) Antibody

catalog :

AMR-061

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 7

Reference
Vieira Rocha M, Rodríguez Rodríguez P, Ferreira Duarte M, Faria M, Sousa J, Morato M, et al. Fetal Undernutrition Modifies Vascular RAS Balance Enhancing Oxidative Damage and Contributing to Remodeling. Int J Mol Sci. 2022;23: pubmed publisher
Lindquist K, Belugin S, Hovhannisyan A, Corey T, Salmon A, Akopian A. Identification of Trigeminal Sensory Neuronal Types Innervating Masseter Muscle. Eneuro. 2021;8: pubmed publisher
Gunarathne L, Angus P, Herath C. Blockade of Mas Receptor or Mas-Related G-Protein Coupled Receptor Type D Reduces Portal Pressure in Cirrhotic but Not in Non-cirrhotic Portal Hypertensive Rats. Front Physiol. 2019;10:1169 pubmed publisher
Bautzová T, Hockley J, Pérez Berezo T, Pujo J, Tranter M, Desormeaux C, et al. 5-oxoETE triggers nociception in constipation-predominant irritable bowel syndrome through MAS-related G protein-coupled receptor D. Sci Signal. 2018;11: pubmed publisher
Patil M, Hovhannisyan A, Akopian A. Characteristics of sensory neuronal groups in CGRP-cre-ER reporter mice: Comparison to Nav1.8-cre, TRPV1-cre and TRPV1-GFP mouse lines. PLoS ONE. 2018;13:e0198601 pubmed publisher
Byers M, Cornel L. Multiple complex somatosensory systems in mature rat molars defined by immunohistochemistry. Arch Oral Biol. 2018;85:84-97 pubmed publisher
Albee L, Eby J, Tripathi A, Laporte H, Gao X, Volkman B, et al. ?1-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc. 2017;6: pubmed publisher

image

image 1 :

Western blot analysis of rat dorsal root ganglion (lanes 1 and 4), mouse testis (lanes 2 and 5) and rat testis (lanes 3 and 6) lysates: - 1-3. Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061), (1:400).4-6. Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD/GPCR TGR7 Blocking Peptide (#BLP-MR061).

image 2 :

Expression of MRGD in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) sections using Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061), (1:200), followed by goat-anti rabbit-AlexaFluor-488. MRGD staining (green) appears in several DRGs (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AMR-061

SKU :

AMR-061-CF_0.2 ml

Product Name :

Anti-MRGPRD (GPCR TGR7) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7TN41

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MR061

Homology :

Mouse - identical It will not recognize MRGPRD from human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD

Product Page - Scientific background :

Mas-related G-protein coupled receptor member D (MRGPRD) is expressed in the dorsal root ganglia, specifically in non-peptidergic, small-diameter, IB4+ C-fiber neurons that innervate the outer layers of the mammalian skin. They transmit polymodal nociceptive information from the skin epidermis to the substantia gelatinosa of the spinal cord.MRGPRD receptor expressing neurons are essential for the full expression of mechanical nociception. Thus, MRGPRD represents an attractive therapeutic target for the development of agents for treating pain1,2.The Mrg family in mice is comprised of six single-copy genes: MrgD, MrgE, MrgF/RTA, MrgG, MrgH/GPR90, and MAS1. In addition, there are three large subfamilies: MrgA, MrgB,and MrgC3.MRGPRD receptor is activated by β-alanine and is able to inhibit KCNQ/M-type potassium channels and to increase excitability of sensory neurons4,5. MRGPRD has the ability to form heterodimers and is encoded by the TGR7 gene. This receptor is co-expressed with two nociceptors, including purinergic receptor (P2X3) and a vanilloid receptor (VR1)5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor

Short Description :

A Rabbit Polyclonal Antibody to MRGPRD (GPCR TGR7) Receptor

Long Description :

Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRD from rat and mouse samples. The antibody will not recognize MRGPRD from human samples.

Negative Control :

BLP-MR061

Positive Control :

Synonyms :

Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MRGPRD

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage