Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody | Alomone Labs AMR-061-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody

catalog :

AMR-061-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Gunarathne L, Angus P, Herath C. Blockade of Mas Receptor or Mas-Related G-Protein Coupled Receptor Type D Reduces Portal Pressure in Cirrhotic but Not in Non-cirrhotic Portal Hypertensive Rats. Front Physiol. 2019;10:1169 pubmed publisher

image

image 1 :

Western blot analysis of rat dorsal root ganglion lysates: - 1. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061-GP), (1:500). 2. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD (GPCR TGR7) Blocking Peptide (BLP-MR061).

product information

CAT :

AMR-061-GP

SKU :

AMR-061-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7TN41

Applications :

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-MR061

Homology :

Mouse - identical It will not recognize MRGPRD from human samples

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD

Product Page - Scientific background :

Mas-related G-protein coupled receptor member D (MRGPRD) is expressed in the dorsal root ganglia, specifically in non-peptidergic, small-diameter, IB4+ C-fiber neurons that innervate the outer layers of the mammalian skin. They transmit polymodal nociceptive information from the skin epidermis to the substantia gelatinosa of the spinal cord.MRGPRD receptor expressing neurons are essential for the full expression of mechanical nociception. Thus, MRGPRD represents an attractive therapeutic target for the development of agents for treating pain1,2.The Mrg family in mice is comprised of six single-copy genes: MrgD, MrgE, MrgF/RTA, MrgG, MrgH/GPR90, and MAS1. In addition, there are three large subfamilies: MrgA, MrgB,and MrgC3.MRGPRD receptor is activated by β-alanine and is able to inhibit KCNQ/M-type potassium channels and to increase excitability of sensory neurons4,5. MRGPRD has the ability to form heterodimers and is encoded by the TGR7 gene. This receptor is co-expressed with two nociceptors, including purinergic receptor (P2X3) and a vanilloid receptor (VR1)5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor

Short Description :

A Guinea Pig Polyclonal Antibody to MRGPRD (GPCR TGR7) Receptor

Long Description :

Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061-GP, formerly AGP-272) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot applications. It has been designed to recognize MRGPRD (GPCR TGR7) from rat and mouse samples. The antibody will not recognize MRGPRD from human samples. The antigen used to immunize guinea pigs is the same as Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-MR061

Positive Control :

Synonyms :

Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MRGPRD

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500-1:800

more info or order :

Alomone Labs product webpage