Anti-MCHR1 (extracellular) Antibody | Alomone Labs AMR-041 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MCHR1 (extracellular) Antibody

catalog :

AMR-041

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Shang M, Cappellesso F, Amorim R, Serneels J, Virga F, Eelen G, et al. Macrophage-derived glutamine boosts satellite cells and muscle regeneration. Nature. 2020;587:626-631 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human SH-SY5Y brain neuroblastoma (lanes 3 and 6) lysates: - 1-3. Anti-MCHR1 (extracellular) Antibody (#AMR-041), (1:200).4-6. Anti-MCHR1 (extracellular) Antibody, preincubated with MCHR1 (extracellular) Blocking Peptide (#BLP-MR041).

image 2 :

Peptide CMDLQTSLLSTGPNASN corresponding to amino acid residues 7-20 of rat MCHR1 (AccessionP97639). Extracellular N-terminus.

product information

CAT :

AMR-041

SKU :

AMR-041-CF_0.2 ml

Product Name :

Anti-MCHR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97639

Applications :

IC IF LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MR041

Homology :

Mouse - 16/17 amino acid residues identical; human - 13/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CMDLQTSLLSTGPNASN, corresponding to amino acid residues 7-20 of rat MCHR1

Product Page - Scientific background :

Melanin-concentrating hormone (MCH) is a 19 amino acid peptide originally isolated from the salmon pituitary gland (in fish the length of the peptide is 17 amino acids)1. In rodents and humans the peptide is synthesized in the brain and is involved in a number of physiological effects, although it is best known for its central role in the control of appetite control. For example, central administration of MCH in mice leads to obesity and conversely, knockout of the MCH gene in mice yields lean animals with an increased metabolic rate and reduced body fat2,3.MCH exerts its effects by binding MCHR1 and MCHR2, two receptors belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members of the family, MCHR1 and MCHR2 have seven transmembrane domains, an extracellular N-terminal and intracellular C-terminal tails. MCHR1 and MCHR2 activation both lead to an increase in intracellular Ca2+ concentration3.In rat, MCHR1 expression is quite broad, and is strongly detected in the brain. Expression of the receptor is also seen in the eye, skeletal muscle, tongue, pituitary, intestine and lymphocytes4,5. MCHR1 is also expressed in adipose tissue where it regulates leptin secretion3. In human, MCHR1 is mostly localized to the brain and pituitary. MCHR2 displays similar localization to that of MCHR1 in the brain5,6. As MCHR2 is a pseudogene in rodents, studying its function is quite challenging. In the brain, MCHR1 is localized in many areas that are known to be involved in various psychological disorders. Indeed, MCHR1-/- animals display antianxiety- and antidepressant-like behavior7,8. Therefore, antagonizing the activity of MCHR1 could indeed serve as a means to treat depression and obesity9.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1

Short Description :

A Rabbit Polyclonal Antibody to Melanin-Concentrating Hormone Receptor 1

Long Description :

Anti-MCHR1 (extracellular) Antibody (#AMR-041) is a highly specific antibody directed against an epitope of the rat Melanin-concentrating hormone receptor 1. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize MCHR1 from rat, mouse, and human samples.

Negative Control :

BLP-MR041

Positive Control :

Synonyms :

Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MCHR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage