Anti-Melatonin Receptor 1B (MTNR1B) Antibody | Alomone Labs AMR-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Melatonin Receptor 1B (MTNR1B) Antibody

catalog :

AMR-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig s2b	Jilg A, Bechstein P, Saade A, Dick M, Li T, Tosini G, et al. Melatonin modulates daytime-dependent synaptic plasticity and learning efficiency. J Pineal Res. 2019;66:e12553 pubmed publisher
	Liu W, Jiang H, Liu X, Hu S, Li H, Feng Y, et al. Melatonin Abates TMJOA Chronic Pain by MT2R in Trigeminal Ganglion Neurons. J Dent Res. 2021;:220345211026551 pubmed publisher
	Xiang J, Zhu W, Yang F, Yu Z, Cai M, Li X, et al. Melatonin-induced ApoE expression in mouse astrocytes protects endothelial cells from OGD-R induced injuries. Transl Psychiatry. 2020;10:181 pubmed publisher
	Xu Z, Wu Y, Zhang Y, Zhang H, Shi L. Melatonin activates BKCa channels in cerebral artery myocytes via both direct and MT receptor/PKC-mediated pathway. Eur J Pharmacol. 2019;842:177-188 pubmed publisher
	Qiu F, Liu X, Zhang Y, Wu Y, Xiao D, Shi L. Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin. Hypertens Res. 2018;41:718-729 pubmed publisher
	Sánchez Bretaño A, Baba K, Janjua U, Piano I, Gargini C, Tosini G. Melatonin partially protects 661W cells from H2O2-induced death by inhibiting Fas/FasL-caspase-3. Mol Vis. 2017;23:844-852 pubmed

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-Melatonin Receptor 1B (MTNR1B) Antibody (#AMR-032), (1:200).3,4. Anti-Melatonin Receptor 1B (MTNR1B) Antibody, preincubated with Melatonin Receptor 1B/MTNR1B Blocking Peptide (#BLP-MR032).

image 2 :

Multiplex staining of Melatonin receptor type 1B and NaV1.2 in rat paraventricular nucleus - Immunohistochemical staining of perfusion-fixed frozen rat paraventricular nucleus sections using Anti-Melatonin Receptor 1B (MTNR1B) Antibody (#AMR-032), (1:600) and Guinea pig Anti-SCN2A (NaV1.2) Antibody (#ASC-002-GP), (1:2000). A. MT2 staining (red) (arrows). B. The same section labeled for NaV1.2 (green). C. Merge of A and B demonstrates partial co-localization of MT2 and NaV1.2 in the paraventricular nucleus (PVN). For orientation, note location with respect to the third ventricle (3rd V). Cell nuclei were stained with DAPI (blue).

product information

CAT :

AMR-032

SKU :

AMR-032-CF_0.2 ml

Product Name :

Anti-Melatonin Receptor 1B (MTNR1B) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8CIQ6

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MR032

Homology :

Rat - 14 /15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RKAKATRKLRLRPSD, corresponding to amino acid residues 232-246 of mouse MTNR1B

Product Page - Scientific background :

Melatonin (N-acetyl-5-methoxytryptamine) is a product of tryptophan metabolism. It is synthesized in the pineal gland and is secreted to control the circadian rhythm1-3. Melatonin is also synthesized in the gastrointestinal tract, retina, skin and other tissues where it acts in a autocrine or paracrine manner. The role of melatonin in these tissues is independent of its role in the circadian rhythm, where it plays a role in energy metabolism, physiological growth, differentiation and responsiveness in stress stimuli1. The pleiotropic effects of melatonin have given rise to various therapeutic possibilities for this molecule. For example; anti-stress, sexual dysfunction, obesity, gallbladder stones1,4. To date, the only therapeutic uses for melatonin remain to treat sleep disorders, depression, migraine and headaches1.Melatonin exerts its effects through two G-protein coupled receptors (GPCRs); melatonin receptor type 1 and melatonin receptor type 2 (MT1 and MT2). Like all GPCRs, they have seven transmembrane domains and extracellular N-terminal and cytoplasmic C-terminal tails. The binding of melatonin to either receptor activates Gi, thereby activating PLC, thus increasing intracellular Ca2+ levels. Both receptors structurally bind melatonin in the same manner, although MT2 displays a much higher affinity for the hormone. Just like melatonin levels are detected in many tissues, the expression patterns of the two receptors are also quite broad. For example, MT1 is detected in the brain, retina and kidneys and MT2 is expressed in brain and in the retina1.MT1 is involved in sleep regulation and might also have effects on peripheral vasoconstriction. MT2 may play an important physiological role in the retina and might regulate body temperature1.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Melatonin receptor type 2, MT2, Mel1b receptor, Mel-1B-R

Short Description :

A Rabbit Polyclonal Antibody to Melatonin Receptor Type 1B

Long Description :

Anti-Melatonin Receptor 1B (MTNR1B) Antibody (#AMR-032) is a highly specific antibody directed against an epitope of mouse MT2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MT2 from rat and mouse samples. The antibody is unlikely to recognize the receptor in human samples.

Negative Control :

BLP-MR032

Positive Control :

Synonyms :

Melatonin receptor type 2, MT2, Mel1b receptor, Mel-1B-R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MTNR1B

AB Specifictiy :

Unlikely to recognize human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600-1:2000

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage