Anti-MC5 Receptor Antibody | Alomone Labs AMR-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MC5 Receptor Antibody

catalog :

AMR-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Lonati C, Battistin M, Dondossola D, Bassani G, Brambilla D, Merighi R, et al. NDP-MSH treatment recovers marginal lungs during ex vivo lung perfusion (EVLP). Peptides. 2021;141:170552 pubmed publisher
Chen H, Li X, Ma H, Zheng W, Shen X. Reduction in Nesfatin-1 Levels in the Cerebrospinal Fluid and Increased Nigrostriatal Degeneration Following Ventricular Administration of Anti-nesfatin-1 Antibody in Mice. Front Neurosci. 2021;15:621173 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-MC5 Receptor Antibody (#AMR-025), (1:200).3,4. Anti-MC5 Receptor Antibody, preincubated with MC5 Receptor Blocking Peptide (#BLP-MR025).

image 2 :

Expression of MC5R in rat skin - Immunohistochemical staining of paraffin embedded rat skin section using Anti-MC5 Receptor Antibody (#AMR-025), (1:100). MC5R staining is present in sebaceous glands. Hematoxilin is used as the counterstain.

image 3 :

Expression of MC5R in rat skin - Immunohistochemical staining of paraffin embedded rat skin section using Anti-MC5 Receptor Antibody(#AMR-025) (1:100). MC5R staining is present in sebaceous glands. Hematoxilin is used as the counterstain.

product information

CAT :

AMR-025

SKU :

AMR-025-CF_0.2 ml

Product Name :

Anti-MC5 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P41149

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MR025

Homology :

Rat - 12/13 amino acid residues identical; human - 7/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RYNSVRQRTSMKG, corresponding to amino acid residues 224-236 of mouse MC5R

Product Page - Scientific background :

Melanocortin receptor 5 (MC5R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G-Protein Coupled Receptor (GPCR) superfamily.The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC) 1,2,3.One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP) 1.All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC5R activation is α-MSH > ACTH = β-MSH >> γ-MSH while AGRP works as an endogenous antagonists of MC5R1,2,3.MC5R is mostly expressed in peripheral tissues such as skeletal muscle, bone marrow, skin tissues particularly in sebaceous glands and exocrine glands such as salivary glands1.The best understood function of Melanocortin Receptor 5 is in the regulation of exocrine glands since MC5R-deficient mice exhibit severely defective water repulsion and thermoregulation due to decreased production of sebaceous lipids4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Melanocortin receptor 5, MC5R, MC5-R

Short Description :

A Rabbit Polyclonal Antibody to Melanocortin Receptor 5

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse melanocortin receptor 5. Anti-MC5 Receptor Antibody (#AMR-025) can be used in western blot and immunohistochemical applications. It has been designed to recognize MC5R from mouse, human and rat samples.

Negative Control :

BLP-MR025

Positive Control :

Synonyms :

Melanocortin receptor 5, MC5R, MC5-R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MC5R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage