Anti-MC4 Receptor (extracellular) Antibody | Alomone Labs AMR-024 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MC4 Receptor (extracellular) Antibody

catalog :

AMR-024

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Xu Y, Jiang Z, Li H, Cai J, Jiang Y, Otiz Guzman J, et al. Lateral septum as a melanocortin downstream site in obesity development. Cell Rep. 2023;42:112502 pubmed publisher
Chen H, Li X, Ma H, Zheng W, Shen X. Reduction in Nesfatin-1 Levels in the Cerebrospinal Fluid and Increased Nigrostriatal Degeneration Following Ventricular Administration of Anti-nesfatin-1 Antibody in Mice. Front Neurosci. 2021;15:621173 pubmed publisher
Avegno E, Lobell T, Itoga C, Baynes B, Whitaker A, Weera M, et al. Central Amygdala Circuits Mediate Hyperalgesia in Alcohol-Dependent Rats. J Neurosci. 2018;38:7761-7773 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysates: - 1. Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:400).2. Anti-MC4 Receptor (extracellular) Antibody, preincubated with MC4 Receptor (extracellular) Blocking Peptide (#BLP-MR024).

image 2 :

Expression of MC4R in mouse brain - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:100). MC4R (green) is expressed in the mouse hypothalamus in axonal processes (arrows). Hoechst 33342 is used as the counterstain (blue).

image 3 :

Western blot analysis of mouse brain lysates: - 1. Anti-MC4 Receptor (extracellular) Antibody(#AMR-024) (1:400).2. Anti-MC4 Receptor (extracellular) Antibody preincubated with the control peptide antigen.

product information

CAT :

AMR-024

SKU :

AMR-024-CF_0.2 ml

Product Name :

Anti-MC4 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32245

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MR024

Homology :

Rat, mouse - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)HRGMHTSLHLWNRSS, corresponding to amino acid residues 6-20 of human MC4R

Product Page - Scientific background :

Melanocortin receptor 4 (MC4R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).1All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC4R activation is α-MSH = ACTH > β-MSH >> γ-MSH. AGRP and agouti/ASP are both endogenous antagonists of MC4R.1,2,3MC4R is widely expressed in the brain including the cortex, thalamus, hypothalamus, and brain stem. The best understood physiological role of MC4R is in the regulation of feeding behavior and the control of body weight. Indeed, mice with a targeted disruption of the MC4R gene display an obese phenotype characterized by increased adiposity, hyperphagia, and hyperleptinaemia.4 Similarly, MC4R mutations in humans have been associated with severe childhood obesity including characteristics very similar to the MC4R knockout mouse phenotype.5 All these make MC4R an attractive drug target for the treatment of obesity.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Melanocortin receptor 4, MC4R, MC4-R

Short Description :

A Rabbit Polyclonal Antibody to Melanocortin Receptor 4

Long Description :

Anti-MC4 Receptor (extracellular) Antibody (#AMR-024) is a highly specific antibody directed against an epitope of the human melanocortin receptor 4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It recognizes an extracellular epitope and can potentially recognize the receptor in living cells. It has been designed to recognize MC4R from human, mouse, and rat samples.

Negative Control :

BLP-MR024

Positive Control :

Synonyms :

Melanocortin receptor 4, MC4R, MC4-R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MC4R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage