antibody

Anti-MC3 Receptor (extracellular) Antibody

AMR-023

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

AMR-023-CF_0.2 ml

Anti-MC3 Receptor (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P32244

IF IFC IHC LCI WB

Human Rat Mouse

Rabbit

BLP-MR023

Mouse - identical; human 13/16 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

1st extracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)NSDSLTLEDQFIQHMD, corresponding to amino acid residues 102-117 of rat MC3R

Melanocortin receptor 3 (MC3R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the salient features of the melanocortin signaling system is the presence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).1All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with differing affinities. MC3R is the only one of the melanocortin receptors that shows no difference in binding specificity for any of the melanocortins, binding all of them with equal efficiency. AGRP is the high affinity endogenous antagonist of MC3R.1,2,3The physiological function of MC3R is still poorly understood. The distribution of MC3R in hypothalamic nuclei of the central nervous system (CNS) suggests a role in the regulation of energy homeostasis. Indeed, studies with MC3R knockout (MC3R KO) mice showed that these mice have increased body fat due to increased feed efficiency.4 In humans, several polymorphisms of the MC3R gene are associated with high insulin levels and obesity in children.5 In addition, expression of MC3R in macrophages is thought to mediate the well known anti-inflammatory effects of α-MSH.6

IF IFC IHC LCI WB

Alomone Labs

Melanocortin receptor 3, MC3R, MC3-R

A Rabbit Polyclonal Antibody to Melanocortin Receptor 3

Anti-MC3 Receptor (extracellular) Antibody (#AMR-023) is a highly specific antibody directed against an epitope of the rat melanocortin receptor 3. The antibody can be used in western blot, immunohistochemistry, and flow cytometry applications. It has been designed to recognize MC3R from human, mouse, and rat samples.

BLP-MR023

NA

Melanocortin receptor 3, MC3R, MC3-R

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

MC3R

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:100

1:200