Anti-MC2 Receptor (extracellular) Antibody | Alomone Labs AMR-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MC2 Receptor (extracellular) Antibody

catalog :

AMR-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat adrenal lysate: - 1. Anti-MC2 Receptor (extracellular) Antibody (#AMR-022), (1:400).2. Anti-MC2 Receptor (extracellular) Antibody, preincubated with MC2 Receptor (extracellular) Blocking Peptide (#BLP-MR022).

image 2 :

Expression of MC2R in rat adrenal gland - Immunohistochemical staining of paraffin embedded rat adrenal cortex section using Anti-MC2 Receptor (extracellular) Antibody (#AMR-022), (1:100). Shown are the capsule (C), the zona glomerulosa (ZG) and the zona fasciculata (ZF) of the adrenal cortex. Note that the staining (brown) is specific for cells of the adrenal gland while no staining is present in the dense connective tissue of the capsule. Color reaction was obtained with DAB. H&E is used as the counterstain.

image 3 :

Expression of MC2R in rat adrenal gland - Immunohistochemical staining of paraffin embeddedrat adrenal cortex section using Anti-MC2 Receptor (extracellular) Antibody(#AMR-022) (1:100).Shown arethe capsule (C) thezona glomerulosa(ZG) and thezona fasciculata(ZF) of the adrenal cortex. Note that the staining (brown) is specific for cells of the adrenal gland while no staining is present in the dense connective tissue of the capsule. Color reaction was obtained with DAB. H E is used as the counterstain.

product information

CAT :

AMR-022

SKU :

AMR-022-CF_0.2 ml

Product Name :

Anti-MC2 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01718

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MR022

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RNMGYLKPRGSFE, corresponding to amino acid residues 87-99 of human MC2R

Product Page - Scientific background :

Melanocortin receptor 2 (MC2R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7 transmembrane domain, G-protein coupled receptor (GPCR) superfamily.The receptors' ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, β- and γ melanocyte stimulating hormone (α, β, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists; proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.MC2R can also be described as the ACTH receptor as it is exclusively activated by the ACTH hormone. In fact, MC2R mediates all the physiological effects of ACTH in the adrenal cortex, that is, the regulation and production of steroids, their circadian variation and their stress-related fluctuations.1,2,3 In addition, mutations that result in MC2R functional impairment are associated with familial glucocorticoid deficiency.4The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Melanocortin receptor 2, MC2R, MC2-R, Adrenocorticotropic hormone receptor, ACTH receptor, ACTHR, ACTH-R, Adrenocorticotropin receptor

Short Description :

A Rabbit Polyclonal Antibody to Melanocortin Receptor 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against the first extracellular loop of the human melanocortin receptor 2. Anti-MC2 Receptor (extracellular) Antibody (#AMR-022) can be used in western blot and immunohistochemistry applications. It has been designed to recognize MC2R from human, mouse, and rat samples.

Negative Control :

BLP-MR022

Positive Control :

Synonyms :

Melanocortin receptor 2, MC2R, MC2-R, Adrenocorticotropic hormone receptor, ACTH receptor, ACTHR, ACTH-R, Adrenocorticotropin receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MC2R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage