Anti-MC1 Receptor Antibody | Alomone Labs AMR-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MC1 Receptor Antibody

catalog :

AMR-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Saporiti F, Piacentini L, Alfieri V, Bono E, Ferrari F, Chiesa M, et al. Melanocortin-1 Receptor Positively Regulates Human Artery Endothelial Cell Migration. Cell Physiol Biochem. 2019;52:1339-1360 pubmed publisher

image

image 1 :

Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6): - 1,2,3. Anti-MC1 Receptor Antibody (#AMR-020), (1:500).4,5,6. Anti-MC1 Receptor Antibody, preincubated with MC1 Receptor Blocking Peptide (#BLP-MR020).

image 2 :

Expression of MC1R in normal skin and melanoma - Immunohistochemical staining of paraffin embedded normal skin and melanoma sections using Anti-MC1 Receptor Antibody (#AMR-020) (1:100). MCR1 staining (red-brown color is highly specific in A. epidermal cells, B. eccrine sweat gland cells and C. melanoma cells. Color reaction was obtained with DAB. Hematoxilin is used as the counterstain.

image 3 :

Peptide (C)HAQGIARLHKRQRPVH corresponding to amino acid residues 217-232 of human MC1R (AccessionQ01726). 3rdintracellular loop.

product information

CAT :

AMR-020

SKU :

AMR-020-CF_0.2 ml

Product Name :

Anti-MC1 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01726

Applications :

IC IF IHC WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MR020

Homology :

Rat - 11/6 amino acids residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)HAQGIARLHKRQRPVH, corresponding to amino acid residues 217-232 of human MC1R

Product Page - Scientific background :

Melanocortin receptor 1 (MC1R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The receptors ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, β- and γ-melanocyte stimulating hormone (α, β, γ-MSH) and the adrenocorticotropic hormone (ACTH) all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, that is proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.MC1R was the first member of the melanocortin receptor family to be cloned. The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP. MC1R binds its ligands with the following potency: α-MSH = ACTH > β-MSH > γ-MSH. MC1R also binds the endogenous antagonist agouti with high affinity.1, 2MC1R can be described as the "classical" melanocyte α-MSH receptor. The receptor is expressed in the skin where it has a key role in determining skin and hair pigmentation. In fur-bearing mammals, the local ratio between α-MSH and agouti will determine coat pigmentation as α-MSH stimulates and agouti inhibits melanin production.In humans, especially in Caucasians, the MC1R gene is highly polymorphic and several allelic variants have been correlated with red-hair, poor tanning ability and increased risk of melanoma.1,3,5 In addition, MC1R expression in melanoma has been shown to be upregulated up to 20-fold when compared to normal melanocytes.The melanocortins and particularly α-MSH have significant anti-inflammatory properties. Since α-MSH binds to MC1R with the highest potency, it was proposed that the latter mediated the anti-inflammatory effects of α-MSH. Indeed, MC1R expression has been demonstrated in several cells of the immune system including macrophages and neutrophils.6

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2

Short Description :

A Rabbit Polyclonal Antibody to Melanocortin Receptor 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human melanocortin receptor 1. Anti-MC1 Receptor Antibody (#AMR-020) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize MC1R from human and rat samples. The antibody is unlikely to recognize MC1R from mouse samples.

Negative Control :

BLP-MR020

Positive Control :

Synonyms :

Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MC1R

AB Specifictiy :

Probably won’t recognize mouse.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400-1:500

more info or order :

Alomone Labs product webpage