Anti-CHRM1 (443-458) Antibody | Alomone Labs AMR-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CHRM1 (443-458) Antibody

catalog :

AMR-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Gillet C, Kurth S, Kuenzel T. Muscarinic modulation of M and h currents in gerbil spherical bushy cells. PLoS ONE. 2020;15:e0226954 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-CHRM1 (443-458) Antibody (#AMR-010), (1:200).2. Anti-CHRM1 (443-458) Antibody, preincubated with CHRM1 (443-458) Blocking Peptide (#BLP-MR010).

image 2 :

Expression of Muscarinic acetylcholine receptor M1 in mouse striatum - Immunohistochemical staining of mouse striatum using Anti-CHRM1 (443-458) Antibody (#AMR-010), (1:100). M1 mAChR staining (red) appears in the matrix (M), not in the patches (P). Blood vessels in the matrix (arrow) are also stained. DAPI is used as the counterstain (blue).

product information

CAT :

AMR-010

SKU :

AMR-010-CF_0.2 ml

Product Name :

Anti-CHRM1 (443-458) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P11229

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MR010

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RKIPKRPGSVHRTPSR, corresponding to amino acid residues 443-458 of human M1 muscarinic receptor

Product Page - Scientific background :

The action of the neurotransmitter acetylcholine (ACh) is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named M1-M5.1-2The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exert both excitatory and inhibitory control over central and peripheral tissues.1-2Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control.1 They also participate in learning and memory processing.3-4 The M1 receptors are the most abundant muscarinic subtype in the cortex and striatum. M1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of ACh from the nerves.5-6The M1, M3 and M5 receptors, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the M1, M3 and M5 receptors is an essential element for the anti-apoptotic response of those receptors.7

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1

Short Description :

A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1

Long Description :

Anti-CHRM1 (443-458) Antibody (#AMR-010) is a highly specific antibody directed against an epitope of the human M1 muscarinic receptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize M1 receptor from mouse, rat, and human samples.

Negative Control :

BLP-MR010

Positive Control :

Synonyms :

Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRM1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage