Anti-CHRM4 Antibody | Alomone Labs AMR-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CHRM4 Antibody

catalog :

AMR-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Wang J, Zhao M, Jia P, Liu F, Chen K, Meng F, et al. The analgesic action of larixyl acetate, a potent TRPC6 inhibitor, in rat neuropathic pain model induced by spared nerve injury. J Neuroinflammation. 2020;17:118 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 2), mouse brain membrane (lane 3 and 5) and SH-SY5Y cell lysate (lanes 4 and 6): - 1,3,4. Anti-CHRM4 Antibody (#AMR-004), (1:200).2,5,6. Anti-CHRM4 Antibody, preincubated with CHRM4 Blocking Peptide (#BLP-MR004).

image 2 :

Expression of Muscarinic acetylcholine receptor M4 in mouse islands of Calleja - Immunohistochemical staining of mouse islands of Calleja (ventral striatum) using Anti-CHRM4 Antibody (#AMR-004). A. M4 mAChR staining (red) appears in soma of neuronal profiles (vertical arrows) and a diffuse halo (horizontal arrows) in the vicinity of the islands of Calleja (IC). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.

image 3 :

Expression of Muscarinic acetylcholine receptorM4in C6 transfected cells - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized C6 cells. A. Transfected cells were stained usingAnti-CHRM4 Antibody(#AMR-004) (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Non-transfected cells were stained using Anti-CHRM4 Antibody (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). DAPI is used as the counterstain (blue).

product information

CAT :

AMR-004

SKU :

AMR-004-CF_0.2 ml

Product Name :

Anti-CHRM4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08485

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-MR004

Homology :

Mouse - identical; human - 9/12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

Product Page - Scientific background :

Acetylcholine is a neurotransmitter which activates two different groups of receptors: nicotinic acetylcholine receptors (nAChR) which belong to the superfamily of ligand-gated ion channels, and muscarinic acetylcholine receptors (mAChR) which belong to the G-protein coupled receptor (GPCR) superfamily. There are five separate gene products (M1-M5) that, assembled, make up this GPCR subfamily.Generally, M1, M3 and M5 muscarinic receptors are known to activate phospholipase C (PLC) via Gq coupling, while M2 and M4 muscarinic receptors couple to Gi/o, and therefore inhibit adenylate cyclase1. This classification is however not clear cut. mAChR can activate adenylate cyclase by coupling to Gs2. In addition, M2 and M4 muscarinic receptors, when overexpressed, can also activate adenylate cyclase in some systems3-5.Evidence suggests that muscarinic receptors can form homo- or heterodimers and that the dimer formed can subsequently affect the downstream signaling pathways1.Muscarinic receptors have been shown to regulate voltage-gated Ca2+ channels, namely CaV2.16, as well acid sensing ion channels (ASIC)7. Generally speaking, the actions of muscarinic receptors on ion channels can be either via 2nd messengers or through their direct action on the channels once activated8.Furthermore, muscarinic receptors can also promote endocytosis of ion channels (KV1.2 for example) by recruiting tyrosine kinases that phosphorylate the channel in order to terminate its activity9. Other diverse and important functions of muscarinic receptors include cell growth, survival and physiology1.Expression of muscarinic receptors is found in neurons in the central nervous system as well as in the peripheral nervous system. These receptors are also expressed in cardiac and smooth muscle, lung, intestine, ovary and urothelium1,10. M4 muscarinic receptor is mostly expressed in the striatum where it colocalizes with D1 dopamine receptors11.Malfunction of M4 muscarinic receptor is associated with a number of psychological and neurodegenerative diseases like Schizophrenia, Alzheimer's and Parkinson's diseases12.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4

Short Description :

A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M4

Long Description :

Anti-CHRM4 Antibody (#AMR-004) is a highly specific antibody directed against an epitope of the rat M4 muscarinic receptor. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize M4 from rat, mouse, and human samples.

Negative Control :

BLP-MR004

Positive Control :

Synonyms :

Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CHRM4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage