Anti-CLEC7A/Dectin-1 (extracellular) Antibody | Alomone Labs ALR-062 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CLEC7A/Dectin-1 (extracellular) Antibody

catalog :

ALR-062

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062), (1:200). 3-4. Anti-CLEC7A/Dectin-1 (extracellular) Antibody, preincubated with CLEC7A/Dectin-1 (extracellular) Blocking Peptide (BLP-LR062).

image 2 :

Expression of CLEC7A/Dectin-1 in rat hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062), (1:2400), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed CLEC7A immunoreactivity (green) in astrocytes of the hilus (H). B. Pre-incubation of the antibody with CLEC7A/Dectin-1 (extracellular) Blocking Peptide (BLP-LR062), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer.

product information

CAT :

ALR-062

SKU :

ALR-062-CF_0.2 ml

Product Name :

Anti-CLEC7A/Dectin-1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6QLQ4

Applications :

IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-LR062

Homology :

Rat - 15 out of 16 amino acid residues identical Not recommended for human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RNKENHKPTESSLDEK, corresponding to amino acid residues 87 - 102 of mouse CLEC7A

Product Page - Scientific background :

Dectin-1, encoded by CLEC7A, is a small transmembrane receptor that is a member of the C-type lectin family of pattern-recognition receptors (PRRs)1,2. As this receptor specifically recognizes and binds to β-glucans polysaccharides derived from the cell walls of fungi and yeast, it has been characterized for its role in antifungal immunity3.Dectin-1 is predominately expressed on macrophages, dendritic cells, and neutrophils4,5. Upon ligation of β-glucan, Dectin-1 recruits the adaptor protein CARD9 to phosphorylate spleen tyrosine kinase (Syk) which in turn initiates a signaling cascade which induces phagocytosis and leads to the activation of nuclear factor-κB (NF-κB) and MAPK6. The Dectin-1 Syk mediated pathway has been demonstrated to be activated and involved in the alternative activation of macrophages as Dectin-1 was upregulated in vitro within 4 hours when stimulated with IL-4 and IL-13, cytokines which induce alternative activation of macrophages7. Moreover, the activated Syk pathway has also been implicated in renal interstitial fibrotic disease where it was demonstrated that suppressing Syk in an experimental mouse model of renal fibrosis inhibits the progression of kidney fibrosis, shown by a reduction in mRNA expression of Arginase-1 (murine M2 macrophage marker)8.While the Dectin-1 pathway has been shown to be involved in the bleomycin-induced model of skin fibrosis, Dectin-1 signaling and receptor expression has yet to be investigated in the context of pulmonary fibrosis9.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369

Short Description :

A Rabbit Polyclonal antibody to CLEC7A/Dectin-1 (extracellular)

Long Description :

Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CLEC7A from rat and mouse samples. The antibody will not recognize CLEC7A from human samples.

Negative Control :

BLP-LR062

Positive Control :

Synonyms :

C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CLEC7A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600-1:2400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage