Anti-Leptin Receptor (extracellular) Antibody | Alomone Labs ALR-039 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Leptin Receptor (extracellular) Antibody

catalog :

ALR-039

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), rat dorsal root ganglion lysate (lanes 2 and 5) and mouse brain membranes (lanes 3 and 6):

image 2 :

Western blot analysis of rat liver lysates:

image 3 :

Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and mouse TK-1 T-cell lymphoma cell line lysate (lanes 2 and 4):

product information

CAT :

ALR-039

SKU :

ALR-039-CF_0.2 ml

Product Name :

Anti-Leptin Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48356

Applications :

IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-LR039

Homology :

Rat - 13 out of 15 amino acid residues identical Won't recognize Leptin Receptor from human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EDDGMKWLRIPSNVK, corresponding to amino acid residues 777 - 791 of mouse LEPR

Product Page - Scientific background :

Leptin receptor (LEP-R), also known as OB receptor (OB-R), HuB219, and CD295 is a receptor for the hormone leptin, which is involved in energy homeostasis and hunger/satiation cues.1LEP-R, encoded by the LEPR gene, is composed of 1165 amino acids with the extracellular domain consisting an N-terminal cytokine receptor homology domain (CRH-1), an immunoglobulin-like (Ig) domain, a second CRH domain (CRH-2) which is also known as the leptin-binding domain (LBD) and two Fibronectin type III (FNIII) domains. LEP-R forms a high-affinity 1:1 ratio complex with leptin in solution, but does not form the 2:1 ratio complex associated with the small cytokine receptors, such as growth hormone receptor (GHR). The 1:1 ratio interaction occurs at leptin's binding site II and can be blocked by mutations in this region. Mutations within the Ig domain of LEP-R and binding site III of leptin have been shown to inhibit transduction without disrupting receptor binding, indicating that the leptin-signaling complex forms a crossover arrangement between two leptin-LEP-R complexes.1Leptin is produced in adipose tissue in proportion to triglyceride stores and serves as an indicator of long-term energy status. Leptin acts mainly in the brain, specifically the hypothalamus, where its action is integrated with that of other adipokines, gastrokines, and other signals to coordinate energy homeostasis. Once secreted into the circulation, leptin travels to the brain where it enters the CNS likely via the choroid plexus and circumventricular organs. In the brain, leptin acts by binding and activating LEP-R, which is primarily expressed on specialized subsets of neurons in certain hypothalamic and brainstem nuclei.2Lack of leptin, such as in cases of starvation or genetic leptin deficiency, increases hunger while promoting an energy-sparing cascade of neuroendocrine and autonomic changes, including decreased sympathetic nervous system tone, thyroid function, growth, reproduction, and a variety of behavioral and physiological changes to respond appropriately to low stores of energy.2

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

LEPR, OB receptor, OBR, CD295

Short Description :

A Rabbit Polyclonal antibody to Leptin Receptor (extracellular)

Long Description :

Anti-Leptin Receptor (extracellular) Antibody (#ALR-039) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Leptin Receptor from mouse and rat samples. The antibody will not work with human samples.

Negative Control :

BLP-LR039

Positive Control :

Synonyms :

LEPR, OB receptor, OBR, CD295

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LEPR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage