antibody

Anti-Leptin Receptor (extracellular)-FITC Antibody

ALR-039-F

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

ALR-039-F_15 mcl

Anti-Leptin Receptor (extracellular)-FITC Antibody

Antibodies

Antibodies

Polyclonal

P48356

FC LCI

Rat Mouse

Rabbit

BLP-LR039

Rat - 13 out of 15 amino acid residues identical Won't recognize Leptin Receptor from human samples

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular, N-terminus.

Fluorescein isothiocyanate (FITC).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)EDDGMKWLRIPSNVK, corresponding to amino acid residues 777 - 791 of mouse LEPR

Leptin receptor (LEP-R), also known as OB receptor (OB-R), HuB219, and CD295 is a receptor for the hormone leptin, which is involved in energy homeostasis and hunger/satiation cues.1LEP-R, encoded by the LEPR gene, is composed of 1165 amino acids with the extracellular domain consisting an N-terminal cytokine receptor homology domain (CRH-1), an immunoglobulin-like (Ig) domain, a second CRH domain (CRH-2) which is also known as the leptin-binding domain (LBD) and two Fibronectin type III (FNIII) domains. LEP-R forms a high-affinity 1:1 ratio complex with leptin in solution, but does not form the 2:1 ratio complex associated with the small cytokine receptors, such as growth hormone receptor (GHR). The 1:1 ratio interaction occurs at leptin's binding site II and can be blocked by mutations in this region. Mutations within the Ig domain of LEP-R and binding site III of leptin have been shown to inhibit transduction without disrupting receptor binding, indicating that the leptin-signaling complex forms a crossover arrangement between two leptin-LEP-R complexes.1Leptin is produced in adipose tissue in proportion to triglyceride stores and serves as an indicator of long-term energy status. Leptin acts mainly in the brain, specifically the hypothalamus, where its action is integrated with that of other adipokines, gastrokines, and other signals to coordinate energy homeostasis. Once secreted into the circulation, leptin travels to the brain where it enters the CNS likely via the choroid plexus and circumventricular organs. In the brain, leptin acts by binding and activating LEP-R, which is primarily expressed on specialized subsets of neurons in certain hypothalamic and brainstem nuclei.2Lack of leptin, such as in cases of starvation or genetic leptin deficiency, increases hunger while promoting an energy-sparing cascade of neuroendocrine and autonomic changes, including decreased sympathetic nervous system tone, thyroid function, growth, reproduction, and a variety of behavioral and physiological changes to respond appropriately to low stores of energy.2

FC LCI

Alomone Labs

LEPR, OB receptor, OBR, CD295

A Rabbit Polyclonal antibody to Leptin Receptor (extracellular) conjugated to the fluorescent dye FITC.

Anti-Leptin Receptor (extracellular) Antibody (#ALR-039) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Leptin Receptor from mouse and rat samples. The antibody will not work with human samples. Anti-Leptin Receptor (extracellular)-FITC Antibody (#ALR-039-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

RIC-001-F

NA

LEPR, OB receptor, OBR, CD295

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

LEPR

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #ALR-039), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody