Anti-LPAR6 (P2RY5) (extracellular) Antibody | Alomone Labs ALR-036 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LPAR6 (P2RY5) (extracellular) Antibody

catalog :

ALR-036

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Choi S, Lee R, Cho H, Hwang S, Hwang H, Rhim H, et al. Visualization of the binding between gintonin, a Panax ginseng-derived LPA receptor ligand, and the LPA receptor subtypes and transactivation of the EGF receptor. J Ginseng Res. 2022;46:348-356 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-LPAR6 (P2RY5) (extracellular) Antibody (#ALR-036), (1:200).3,4. Anti-LPAR6 (P2RY5) (extracellular) Antibody, preincubated with LPAR6/P2RY5 (extracellular) Blocking Peptide (#BLP-LR036).

image 2 :

Peptide STHSQGNNASEAC corresponding to amino acid residues 156-168 of human LPAreceptor 6 (AccessionP43657). 2ndextracellular loop.

image 3 :

Peptide NKSLSSFKENEENIQC corresponding to amino acid residues 84-99 of rat CB1 receptor (AccessionP20272). Extracellular N-terminus.

product information

CAT :

ALR-036

SKU :

ALR-036-CF_0.2 ml

Product Name :

Anti-LPAR6 (P2RY5) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P43657

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-LR036

Homology :

Rat, mouse -12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

STHSQGNNASEAC, corresponding to amino acid residues 156-168 of human LPA receptor 6

Product Page - Scientific background :

Lysophosphatidic acid (LPA; 1- or 2-acyl-sn-glycerol-3-phosphate) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptor have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2.LPA6 (P2Y5) was cloned as an orphan GPCR and is closely related to the fourth lysophosphatidic acid receptor, LPA43. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N terminus is on the extracellular side of the membrane, and the C terminus is on the cytoplasmic side4.LPA6 mRNA is expressed ubiquitously including in skin and hair follicle cells.A recent study suggested that LPA6 is essential for human hair growth. A loss of function is not compensated for, and ultimately leads to pathological changes and hair loss5.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

Lysophosphatidic acid receptor 6, LPA receptor 6, LPA-6, P2Y purinoceptor 5, Purinergic receptor 5, P2Y5, RB intron encoded G-protein coupled receptor

Short Description :

A Rabbit Polyclonal Antibody to LPA Receptor 6 (P2RY5)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human LPA receptor 6. Anti-LPAR6 (P2RY5) (extracellular) Antibody (#ALR-036) can be used in western blot analysis and indirect flow cytometry. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA receptor 6 from mouse, rat and human samples.

Negative Control :

BLP-LR036

Positive Control :

Synonyms :

Lysophosphatidic acid receptor 6, LPA receptor 6, LPA-6, P2Y purinoceptor 5, Purinergic receptor 5, P2Y5, RB intron encoded G-protein coupled receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LPAR6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage