Anti-Human LPAR5 (extracellular) Antibody | Alomone Labs ALR-035 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human LPAR5 (extracellular) Antibody

catalog :

ALR-035

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human chronic myelogenous leukemia MEG-01 (lanes 1 and 3) and K562 cell lysates (lanes 2 and 4): - 1,2. Anti-Human LPAR5 (extracellular) Antibody (#ALR-035), (1:200).3,4. Anti-Human LPAR5 (extracellular) Antibody, preincubated with Human LPAR5 (extracellular) Blocking Peptide (#BLP-LR035).

image 2 :

Peptide (C)RSKLVAASVPARDRVR corresponding to amino acid residues 261-276 of human LPA5receptor (AccessionQ9H1C0).3rdextracellular loop.

image 3 :

Alomone Labs CGP-37849 inhibits NMDA receptor channels expressed in Xenopus oocytes. - A. Representative time course of NR1/NR2A currents elicited by transient stimulations with 50 M glutamate + 50 M glycine every 50 sec while membrane potential was held at -60 mV. 4 min applications of 5 nM and 25 nM CGP-37849 (#C-325) reversibly inhibits current amplitude. B. Superimposed current traces from the recording shown in A upon application of control 5 nM and 25 nM CGP-37849 (as indicated).

product information

CAT :

ALR-035

SKU :

ALR-035-CF_0.2 ml

Product Name :

Anti-Human LPAR5 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H1C0

Applications :

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-LR035

Homology :

Mouse, Rat - not recommended for use with mouse and rat samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RSKLVAASVPARDRVR, corresponding to amino acid residues 261-276 of human LPA5 receptor

Product Page - Scientific background :

LPA (lysophosphatidic acid) mediates cellular differentiation, growth, survival and motility through two subgroups of G-protein coupled receptors (GPCRs): The Edg (endothelial differentiation gene) family - LPA1, LPA2, LPA3 and the non-Edg family - LPA4, LPA5, LPA61.Like all GPCRs, LPA receptors have seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail1.Alkyl-LPA, commonly found in platelets activates LPA5, thus explaining the high expression of the receptor in platelets2. LPA is expressed in astrocytes, and sensory and motor neurons in the spinal cord. It is also detected in spleen, heart, placenta, lliver and colon3.LPA5 activation leads to cAMP production; however, it does so in a Gs independent manner4,5. The receptor couples Gq, thus leading to Ca2+ mobilization4.Activation of LPA5 was also shown to initiate neuropathic pain in mouse models. LPA5 knockout mice do not develop neuropathic pain6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93

Short Description :

A Rabbit Polyclonal Antibody to LPA Receptor 5

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human LPA5 receptor. Anti-Human LPAR5 (extracellular) Antibody (#ALR-035) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA5 receptor from human samples only.

Negative Control :

BLP-LR035

Positive Control :

Synonyms :

Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LPAR5

AB Specifictiy :

Will not recognize rat or mouse LPA5 receptor.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage