Anti-LPAR4 (P2Y9) (extracellular) Antibody | Alomone Labs ALR-034 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LPAR4 (P2Y9) (extracellular) Antibody

catalog :

ALR-034

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes: - 1,2. Anti-LPAR4 (P2Y9) (extracellular) Antibody (#ALR-034), (1:500).3,4. Anti-LPAR4 (P2Y9) (extracellular) Antibody, preincubated with LPAR4/P2Y9 (extracellular) Blocking Peptide (#BLP-LR034).

image 2 :

Expression of LPA4 receptor in rat and mouse brain - Immunohistochemical staining of rat choroid plexus and mouse hippocampal CA3 region using Anti-LPAR4 (P2Y9) (extracellular) Antibody (#ALR-034), (1:400). A. LPA4 receptor staining (red) in rat choroid plexus appears in astrocytes (vertical arrows) and in cells of the choroid plexus (horizontal arrow). C. LPA4 receptor staining (red) in mouse hippocampal CA3 region is detected in astrocytes (arrows). B, D. Nuclear staining using DAPI as the counterstain (blue).

product information

CAT :

ALR-034

SKU :

ALR-034-CF_0.2 ml

Product Name :

Anti-LPAR4 (P2Y9) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BLG2

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-LR034

Homology :

Rat - identical, human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)DRRFIDFQFQDLNS, corresponding to amino acid residues 3-16 of mouse LPA4 receptor

Product Page - Scientific background :

Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptor have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family. LPA4, a member of the purinergic receptor family, and the recently identified LPA5 are structurally distant from the canonical Edg LPA1-3 receptors. LPA4 and LPA5 are linked to Gq, G12/13 and Gs but not Gi, while LPA1-3 all couple to Gi in addition to Gq and G12/132.LPA4 (P2Y9/GPR23) was cloned as an orphan GPCR and is closely related to the purinergic P2Y receptors3. Like all GPCRs it has seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.LPA4 is weakly expressed; there are high levels only in ovary2.LPA4-deficient mice display abnormalities in the blood vascular systems, which caused lethality of embryos and neonates5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, P2Y purinoceptor 9, Purinergic receptor 9, P2RY9, GPR23

Short Description :

A Rabbit Polyclonal Antibody to LPA Receptor 4 (P2Y9)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse LPA4 receptor. Anti-LPAR4 (P2Y9) (extracellular) Antibody (#ALR-034) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA4 receptor from mouse, rat and human samples.

Negative Control :

BLP-LR034

Positive Control :

Synonyms :

Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, P2Y purinoceptor 9, Purinergic receptor 9, P2RY9, GPR23

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LPAR4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage