Anti-LPAR3 (EDG7) (extracellular) Antibody | Alomone Labs ALR-033 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LPAR3 (EDG7) (extracellular) Antibody

catalog :

ALR-033

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Olianas M, Dedoni S, Onali P. LPA1 Mediates Antidepressant-Induced ERK1/2 Signaling and Protection from Oxidative Stress in Glial Cells. J Pharmacol Exp Ther. 2016;359:340-353 pubmed

image

image 1 :

Western blot analysis of rat testis (lanes 1 and 3) and human OVCAR-3 (lanes 2 and 4): - 1,2. Anti-LPAR3 (EDG7) (extracellular) Antibody (#ALR-033), (1:200).3,4. Anti-LPAR3 (EDG7) (extracellular) Antibody, preincubated with LPAR3/EDG7 (extracellular) Blocking Peptide (#BLP-LR033).

image 2 :

Western blot analysis of rat testis (lanes 1 and 3) and human OVCAR-3 (lanes 2 and 4): - 12. Anti-LPAR3 (EDG7) (extracellular) Antibody(#ALR-033) (1:200).34. Anti-LPAR3 (EDG7) (extracellular) Antibody preincubated with the negative control antigen.

image 3 :

Peptide (C)RGPTITDKDRTKGPAE corresponding to amino acid residues 578-593 of rat KCNQ2 (AccessionO88943). Intracellular C-terminus.

product information

CAT :

ALR-033

SKU :

ALR-033-CF_0.2 ml

Product Name :

Anti-LPAR3 (EDG7) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8K5E0

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-LR033

Homology :

Mouse - identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RSNTDTADEWTGTK, corresponding to amino acid residues 16-29 of rat LPA3 receptor

Product Page - Scientific background :

Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptors have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2. All six receptors are type I, rhodopsin-like G-protein coupled receptors (GPCRs) that differ in their tissue distribution and downstream signaling pathways3.GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.Expression of LPA3 receptor has been observed in human heart, testis, prostate, pancreas, lung, ovary, and brain5 and is most abundant in mouse testis, kidney, lung, small intestine, heart, stomach, spleen, brain, and thymus6.LPA3 receptor null mice are viable and grossly normal, but female nulls show a striking phenotype in the reproductive system7. However, despite the fact that LPA3 is expressed in the frontal cortex, hippocampus, and amygdala, no phenotypes related to LPA3 loss in the nervous system have been reported to date5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32

Short Description :

A Rabbit Polyclonal Antibody to LPA Receptor 3 (EDG7)

Long Description :

Anti-LPAR3 (EDG7) (extracellular) Antibody (#ALR-033) is a highly specific antibody directed against an epitope to the rat LPA3 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA3 receptor from mouse, rat, and human samples.

Negative Control :

BLP-LR033

Positive Control :

Synonyms :

Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LPAR3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage