Anti-LPAR2 (EDG4) (extracellular) Antibody | Alomone Labs ALR-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LPAR2 (EDG4) (extracellular) Antibody

catalog :

ALR-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Olianas M, Dedoni S, Onali P. LPA1 Mediates Antidepressant-Induced ERK1/2 Signaling and Protection from Oxidative Stress in Glial Cells. J Pharmacol Exp Ther. 2016;359:340-353 pubmed

image

image 1 :

Western blot analysis of rat colon (lanes 1 and 3) and human colorectal adenocarcinoma (colo205) (lanes 2 and 4) lysates: - 1,2. Anti-LPAR2 (EDG4) (extracellular) Antibody (#ALR-032), (1:200).3,4. Anti-LPAR2 (EDG4) (extracellular) Antibody, preincubated with LPAR2/EDG4 (extracellular) Blocking Peptide (#BLP-LR032).

image 2 :

Western blot analysis of rat colon (lanes 1 and 3) and human colorectal adenocarcinoma (colo205) (lanes 2 and 4) lysates: - 12. Anti-LPAR2 (EDG4) (extracellular) Antibody(#ALR-032) (1:200).34. Anti-LPAR2 (EDG4) (extracellular) Antibody preincubated with the negative control antigen.

image 3 :

Western blot analysis of rat brain membranes (lanes 1 and 3) mouse brain membranes (lanes 2 and 4) and human LNCaP cell lysate (lanes 3 and 6): - 1-3. Anti-QRFPR/GPR103 (extracellular) Antibody(#AGR-033) (1:500).4-6. Anti-QRFPR/GPR103 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ALR-032

SKU :

ALR-032-CF_0.2 ml

Product Name :

Anti-LPAR2 (EDG4) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JL06

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-LR032

Homology :

Rat - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)NSGKELSLHWRPKD, corresponding to amino acid residues 16-29 of mouse LPA2 receptor

Product Page - Scientific background :

Lysophosphatidic acid (LPA) is a lipid mediator that induces various cellular responses including proliferation, migration and inhibition of apoptosis in numerous types of cells via its specific G protein-coupled receptors. At least 5 LPA G-protein coupled receptors (GPCRs) have been identified so far. LPA mediates its effects predominantly by interacting with members of the endothelial differentiation gene (EDG) subfamily of GPCRs: LPA1/EDG2, LPA2/EDG4, and LPA3/EDG71. LPA2 couples to three distinct families of heterotrimeric GTP binding proteins, including Gαq, Gαi, and Gα12/132.LPA2 spans the plasma membrane seven times and contains three extracellular loops and three intracellular loops. LPA2 is unique from the other LPA receptors as it contains two distinct protein-protein interaction domains in the carboxyl-terminal tail (aa 296-351). In the proximal region, LPA2 contains a di-leucine motif and several putative palmitoylated cysteine residues. This region is responsible for associating with zinc-finger proteins3.LPA2 mRNA is expressed in a variety of tissues including human testis, leukocytes, prostate, spleen, thymus, pancreas, and bone marrow3.LPA2-null mice do not show any obvious phenotypic aberrations5. On the other hand, LPA2 expression is increased in ovarian and thyroid cancers and inflammation, suggesting that the main function of LPA2 could become evident under pathophysiological conditions6.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2

Short Description :

A Rabbit Polyclonal Antibody to LPA Receptor 2 (EDG4)

Long Description :

Anti-LPAR2 (EDG4) (extracellular) Antibody (#ALR-032) is a highly specific antibody directed against an epitope of the mouse LPA2 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. It has been designed to recognize LPA2 receptor from human, rat, and mouse samples.

Negative Control :

BLP-LR032

Positive Control :

Synonyms :

Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LPAR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage