Anti-GP130 (extracellular) Antibody | Alomone Labs ALR-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GP130 (extracellular) Antibody

catalog :

ALR-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membrane (lanes 1 and 5), rat brain lysate (lanes 2 and 6), rat brain membrane (lanes 3 and 7) and rat uterus lysate (lanes 4 and 8): - 1-3. Anti-GP130 (extracellular) Antibody (#ALR-023), (1:400).4. Anti-GP130 (extracellular) Antibody (1:200).5-8. Anti-GP130 (extracellular) Antibody, preincubated with GP130 (extracellular) Blocking Peptide (#BLP-LR023).

image 2 :

Expression of GP130/CD130 in rat subfornical organ (SFO). - Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-GP130 (extracellular) Antibody (#ALR-023), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. GP130/CD130 immunoreactivity (green) appears in the SFO (arrows). B. Pre-incubation of the antibody with GP130 (extracellular) Blocking Peptide (BLP-LR023), suppressed staining. Cell nuclei are stained with DAPI (blue). FX = fornix; V = cerebral ventricle.

image 3 :

Expression of ASCT2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections withAnti-ASCT2 (SLC1A5) Antibody(#ANT-082) (1:200) followed by goat-anti-rabbit-AlexaFluor-488. ASCT2 staining appears in a layer (arrows) adjacent to the dentate granule layer (GL). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ALR-023

SKU :

ALR-023-CF_0.2 ml

Product Name :

Anti-GP130 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P40190

Applications :

IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-LR023

Homology :

Mouse, human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DTLYMVHMAAYTEEG, corresponding to amino acid residues 584-598 of rat GP130

Product Page - Scientific background :

Interleukins (ILs) are key mediators in the regulation and coordination of the immune response. Glycoprotein 130 (gp130) belongs to the family of class I cytokine receptors and is the common signal transducing receptor subunit of the IL-6-type cytokines: IL-6, IL-11, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), and cardiotrophin-11.The inflammatory cytokines IL-6 and IL-11 induce gp130 homodimerization after binding to their specific α receptors, which leads to the activation of the Janus kinase/STAT signal transduction pathway2. Gp130 Heterodimerizes with LIFR in response to LIF, CNTF, OSM, or CT13.The extracellular part of gp130 contains an Ig-like domain (D1) followed by single cytokine binding module (CBM) (D2 and D3) and three ﬁbronectin type III-like (FNIII) domains (D4, D5, and D6)3.Gp130 mRNA is ubiquitously expressed in the human body, exhibiting highest levels in saphenous vein, pericardium, ovary, omental adipose tissue, peritoneum, spleen lymph nodes, and trigeminal ganglia4.Dysregulation of gp130 expression, activation, or associated signaling pathways are implicated in a variety of human diseases including cancer, rheumatoid arthritis, Castleman's disease, cardiac myxoma, Crohn's disease, psoriasis, asthma, amyotrophic lateral sclerosis (CNTF), diabetes, atherosclerosis, systemic lupus, obesity, and postmenopausal osteoporosis5.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

Interleukin-6 receptor subunit beta, IL-6R-beta, IL-6RB, Interleukin-6 signal transducer, Oncostatin-M receptor subunit alpha, CD130, Il6st

Short Description :

A Rabbit Polyclonal Antibody to GP130

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat Membrane glycoprotein 130. Anti-GP130 (extracellular) Antibody (#ALR-023) can be used in western blot analysis. It has been designed to recognize GP130 from mouse, rat and human samples.

Negative Control :

BLP-LR023

Positive Control :

Synonyms :

Interleukin-6 receptor subunit beta, IL-6R-beta, IL-6RB, Interleukin-6 signal transducer, Oncostatin-M receptor subunit alpha, CD130, Il6st

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

IL6ST

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage