Anti-LH Receptor (LHCGR) (extracellular) Antibody | Alomone Labs ALR-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LH Receptor (LHCGR) (extracellular) Antibody

catalog :

ALR-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat ovary lysate: - 1. Anti-LH Receptor (LHCGR) (extracellular) Antibody (#ALR-010), (1:2000).2. Anti-LH Receptor (LHCGR) (extracellular) Antibody, preincubated with LH Receptor/LHCGR (extracellular) Blocking Peptide (#BLP-LR010).

image 2 :

Peptide ENELSGWDYDYGFC corresponding to amino acid residues 327-340 of rat LHCGR (AccessionP16235). Extracellular N-terminus.

image 3 :

Expression of LHRin human ovary cell line - Cell surface detection ofLHR in intact living OVCAR3 cells. A. Extracellular staining of cells withAnti-LH Receptor (LHCGR) (extracellular) Antibody(#ALR-010) (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI as the counterstain. C. Merged images of A and B.

product information

CAT :

ALR-010

SKU :

ALR-010-CF_0.2 ml

Product Name :

Anti-LH Receptor (LHCGR) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P16235

Applications :

IC IF LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-LR010

Homology :

Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

ENELSGWDYDYGFC, corresponding to amino acid residues 327-340 of rat LHCGR

Product Page - Scientific background :

The luteinizing hormone receptor (LHR) is a member of the subfamily of glycoprotein hormone receptors within the superfamily of G-protein coupled receptor (GPCR)/seven-transmembrane domain receptors1. The LH/CG receptor is comprised of two structurally and functionally distinct domains, extracellular N-terminal exodomain and membrane-embedded endodomain. These two domains can separately be expressed and processed, including folding. The exodomain alone has the high-affinity hormone binding site but is not capable of generating hormonal signal. In contrast, the endodomain alone has the site for receptor activation2.For many years, LHR was thought to be localized strictly to gonadal cells. In the testes, the LHR is thought to be restricted to Leydig cells. In the ovary, expression of the LHR occurs in theca cells, interstitial cells, differentiated granulosa cells, and luteal cells3. There is increasing evidence that LHR may be present in extragonadal tissues such as: uterus, female reproductive tract tissues, sperm, seminal vesicles, prostate, skin, breast cell lines, lactating mammary gland, adrenals, neural retina, neuroendocrine cells, and brain4.Mutations in the LH receptor have a strong impact on the male phenotype. Activating mutations of the LH receptor leads to male-limited pseudoprecocious puberty, whereas inactivating mutations result in male pseudohermaphroditism4.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Luteinizing hormone receptor, Lutropin-choriogonadotropic hormone receptor, LHCGR, LHR, LSH-R

Short Description :

A Rabbit Polyclonal Antibody to LH Receptor (LHCGR)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat Luteinizing hormone receptor. Anti-LH Receptor (LHCGR) (extracellular) Antibody (#ALR-010) can be used in western blot and live cell imaging applications. It has been designed to recognize LHR from rat, mouse and human samples.

Negative Control :

BLP-LR010

Positive Control :

Synonyms :

Luteinizing hormone receptor, Lutropin-choriogonadotropic hormone receptor, LHCGR, LHR, LSH-R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LHCGR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:2000

more info or order :

Alomone Labs product webpage