Anti-Human CysLTR2 (extracellular) Antibody | Alomone Labs ALR-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human CysLTR2 (extracellular) Antibody

catalog :

ALR-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of acute monocytic leukemia THP-1 (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and chronic myelogenous leukemia K562 (lanes 3 and 6) human cell lysates: - 1-3. Anti-Human CysLTR2 (extracellular) Antibody (#ALR-004), (1:200).4-6. Anti-Human CysLTR2 (extracellular) Antibody, preincubated with Human CysLTR2 (extracellular) Blocking Peptide (#BLP-LR004).

image 2 :

Peptide (C)HLTTWKVGL(S)KDRLH corresponding to amino acid residues 270-284 of human CysLTR2 (AccessionQ9NS75). 3rdextracellular loop.

image 3 :

Cell surface detection ofCysLTR2 in live intact acute monocytic leukemia (THP-1) cells: - ___Control cells + goat anti-rabbit-AlexaFluor-488.___Cells +Anti-Human CysLTR2 (extracellular) Antibody (#ALR-004)(1:50) + goat anti-rabbit-AlexaFluor-488.

product information

CAT :

ALR-004

SKU :

ALR-004-CF_0.2 ml

Product Name :

Anti-Human CysLTR2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9NS75

Applications :

IFC WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-LR004

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)HLTTWKVGL(S)KDRLH, corresponding to amino acid residues 270-284 of human CysLTR2

Product Page - Scientific background :

LTC4, LTD4, and LTE4 are cysteinyl leukotrienes (cys-LTs) peptide conjugated lipids derived from arachidonic acid as a result of immune or inflammatory stimuli1-3.The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their signaling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities5. In mouse, CysLTR1 and CysLTR2 are subject to alternative splicing. CysLTR1 receptor expresses greater affinity for LTD4 and binds equally to LTC4 and LTE4whereas CysLTR2 has greater and equal affinity towards LTC4 and LTD4 over LTE45.Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain4. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spinal cord and adrenal gland differs it from that of CysLTR14.Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestinal, skin and immune disorders1,6.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

CYSLT2, Cysteinyl leukotriene receptor 2, hGPCR21, G-protein coupled receptor HG57

Short Description :

A Rabbit Polyclonal Antibody to Human CysLTR2

Long Description :

Anti-Human CysLTR2 (extracellular) Antibody (#ALR-004) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CysLTR2 from human samples only.

Negative Control :

BLP-LR004

Positive Control :

Synonyms :

CYSLT2, Cysteinyl leukotriene receptor 2, hGPCR21, G-protein coupled receptor HG57

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CYSLTR2

AB Specifictiy :

Not recommended for use with rat and mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage