Anti-Human BLT1 (extracellular) Antibody | Alomone Labs ALR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human BLT1 (extracellular) Antibody

catalog :

ALR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of acute T-cell leukemia Jurkat (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute monocytic leukemia THP-1 (lanes 3 and 6) human cell lysates: - 1-3. Anti-Human BLT1 (extracellular) Antibody (#ALR-001), (1:200).4-6. Anti-Human BLT1 (extracellular) Antibody, preincubated with Human BLT1 (extracellular) Blocking Peptide (#BLP-LR001).

image 2 :

Peptide CFPRYPSEGHRAFH corresponding to amino acid residues 168-181 of human LTB4R (AccessionQ15722). 2ndextracellular loop.

image 3 :

Cell surface detection of LTB4R in live intact human acute monocytic leukemia (THP-1) cell line: - ___Control cells + goat-anti-rabbit-Cy5.___Cells +Anti-Human BLT1 (extracellular) Antibody (#ALR-001) (1:25) + goat-anti-rabbit-Cy5.

product information

CAT :

ALR-001

SKU :

ALR-001-CF_0.2 ml

Product Name :

Anti-Human BLT1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q15722

Applications :

IFC WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-LR001

Homology :

Monkey - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CFPRYPSEGHRAFH, corresponding to amino acid residues 168-181 of human LTB4R

Product Page - Scientific background :

Leukotrienes (LTs) are lipid mediators prominently exerting proinflammatory responses. They are divided in two classes: cysteinyl LTs (cysLTs) which have a thioester linkage and LTB41. LTB4, a chemoattractant, plays a prominent role in the recruitment and activation of leukocytes2.LTB4 acts through two receptors which belong to the G-protein coupled receptor superfamily: BLT1 and BLT2. BLT1 displays high affinity for LTB4 and is highly expressed in leukocytes including granulocytes, macrophages, mast cells, dendritic cells and effector T cells3 and is expressed to much lower levels in the spleen, thymus, heart, skeletal muscle, brain and liver2-4. BLT2 on the other hand is a low affinity receptor which does not display selectivity towards LTB45. Its expression is much more ubiquitous than that of BLT1. It is expressed in most human tissues, with the highest expression being in the spleen, liver, ovary, and leukocytes2,5-8. The two receptors could couple and activate different G-proteins, depending on the cell type and the cellular events activating the receptors2. However, activation of BLT1 usually correlates with increasing intracellular concentrations of Ca2+.The LTB4/BLT1 system is involved in many allergic reactions as well as asthma induced as an allergic response, where it plays a significant role in recruiting neutrophils and effector T cells into lungs as part of an inflammatory response induced by allergens1.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7

Short Description :

A Rabbit Polyclonal Antibody to BLT1

Long Description :

Anti-Human BLT1 (extracellular) Antibody (#ALR-001) is a highly specific antibody directed against an extracellular epitope of the human Leukotriene B4 receptor 1. The antibody can be used for western blot and indirect flow cytometry applications. It has been designed to recognize LTB4R from human samples only.

Negative Control :

BLP-LR001

Positive Control :

Synonyms :

Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

LTB4R

AB Specifictiy :

Not recommended for use with rat and mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage