Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody | Alomone Labs ALM-051 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody

catalog :

ALM-051

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6C1.

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 3a	Chimote A, Gawali V, Newton H, Wise Draper T, Conforti L. A Compartmentalized Reduction in Membrane-Proximal Calmodulin Reduces the Immune Surveillance Capabilities of CD8+ T Cells in Head and Neck Cancer. Front Pharmacol. 2020;11:143 pubmed publisher
immunocytochemistry; mouse; 1:100; fig 3	Li Y, Hu H, Butterworth M, Tian J, Zhu M, O Neil R. Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS ONE. 2016;11:e0155006 pubmed publisher
	Buks R, Dagher T, Rotordam M, Monedero Alonso D, Cochet S, Gautier E, et al. Altered Ca2+ Homeostasis in Red Blood Cells of Polycythemia Vera Patients Following Disturbed Organelle Sorting during Terminal Erythropoiesis. Cells. 2021;11: pubmed publisher
	Centeio R, Ousingsawat J, Schreiber R, Kunzelmann K. CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A. Int J Mol Sci. 2021;22: pubmed publisher
	Li X, Liu Y, Cao A, Li C, Wang L, Wu Q, et al. Crocin Improves Endothelial Mitochondrial Dysfunction via GPx1/ROS/KCa3.1 Signal Axis in Diabetes. Front Cell Dev Biol. 2021;9:651434 pubmed publisher
	Chen X, Zhang J, Song Y, Yang P, Yang Y, Huang Z, et al. Deficiency of anti-inflammatory cytokine IL-4 leads to neural hyperexcitability and aggravates cerebral ischemia-reperfusion injury. Acta Pharm Sin B. 2020;10:1634-1645 pubmed publisher
	Lu J, Dou F, Yu Z. The potassium channel KCa3.1 represents a valid pharmacological target for microgliosis-induced neuronal impairment in a mouse model of Parkinson's disease. J Neuroinflammation. 2019;16:273 pubmed publisher
	Fei Y, Wang Q, Hou J, Li W, Cai X, Yang Y, et al. Macrophages facilitate post myocardial infarction arrhythmias: roles of gap junction and KCa3.1. Theranostics. 2019;9:6396-6411 pubmed publisher
	Wei T, Wang Y, Xu W, Liu Y, Chen H, Yu Z. KCa3.1 deficiency attenuates neuroinflammation by regulating an astrocyte phenotype switch involving the PI3K/AKT/GSK3β pathway. Neurobiol Dis. 2019;132:104588 pubmed publisher
	Jin L, Lucente J, Nguyen H, Singh V, Singh L, Chavez M, et al. Repurposing the KCa3.1 inhibitor senicapoc for Alzheimer's disease. Ann Clin Transl Neurol. 2019;6:723-738 pubmed publisher
	Yu Z, Dou F, Wang Y, Hou L, Chen H. Ca2+-dependent endoplasmic reticulum stress correlation with astrogliosis involves upregulation of KCa3.1 and inhibition of AKT/mTOR signaling. J Neuroinflammation. 2018;15:316 pubmed publisher
	Liu Y, Cole V, Lawandy I, Ehsan A, Sellke F, Feng J. Decreased coronary arteriolar response to KCa channel opener after cardioplegic arrest in diabetic patients. Mol Cell Biochem. 2018;445:187-194 pubmed publisher
	Yu Z, Yi M, Wei T, Gao X, Chen H. KCa3.1 Inhibition Switches the Astrocyte Phenotype during Astrogliosis Associated with Ischemic Stroke Via Endoplasmic Reticulum Stress and MAPK Signaling Pathways. Front Cell Neurosci. 2017;11:319 pubmed publisher
	Yi M, Wei T, Wang Y, Lu Q, Chen G, Gao X, et al. The potassium channel KCa3.1 constitutes a pharmacological target for astrogliosis associated with ischemia stroke. J Neuroinflammation. 2017;14:203 pubmed publisher
	Yu Z, Wang Y, Qin L, Chen H. Functional Cooperation between KCa3.1 and TRPV4 Channels in Bronchial Smooth Muscle Cell Proliferation Associated with Chronic Asthma. Front Pharmacol. 2017;8:559 pubmed publisher
	Paka L, Smith D, Jung D, McCormack S, Zhou P, Duan B, et al. Anti-steatotic and anti-fibrotic effects of the KCa3.1 channel inhibitor, Senicapoc, in non-alcoholic liver disease. World J Gastroenterol. 2017;23:4181-4190 pubmed publisher
	Robles Martínez L, Garay E, Martel Gallegos M, Cisneros Mejorado A, Perez Montiel D, Lara A, et al. Kca3.1 Activation Via P2y2 Purinergic Receptors Promotes Human Ovarian Cancer Cell (Skov-3) Migration. Sci Rep. 2017;7:4340 pubmed publisher
	Yi M, Yu P, Lu Q, Geller H, Yu Z, Chen H. KCa3.1 constitutes a pharmacological target for astrogliosis associated with Alzheimer's disease. Mol Cell Neurosci. 2016;76:21-32 pubmed publisher
	Srivastava S, Panda S, Li Z, Fuhs S, Hunter T, Thiele D, et al. Histidine phosphorylation relieves copper inhibition in the mammalian potassium channel KCa3.1. elife. 2016;5: pubmed publisher
	Zhang P, Yang X, Yin Q, Yi J, Shen W, Zhao L, et al. Inhibition of SK4 Potassium Channels Suppresses Cell Proliferation, Migration and the Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells. PLoS ONE. 2016;11:e0154471 pubmed publisher
	Ohya S, Kanatsuka S, Hatano N, Kito H, Matsui A, Fujimoto M, et al. Downregulation of the Ca(2+)-activated K(+) channel KC a3.1 by histone deacetylase inhibition in human breast cancer cells. Pharmacol Res Perspect. 2016;4:e00228 pubmed publisher
	Grimaldi A, D Alessandro G, Golia M, Grössinger E, Di Angelantonio S, Ragozzino D, et al. KCa3.1 inhibition switches the phenotype of glioma-infiltrating microglia/macrophages. Cell Death Dis. 2016;7:e2174 pubmed publisher
	Crottès D, Felix R, Meley D, Chadet S, Herr F, Audiger C, et al. Immature human dendritic cells enhance their migration through KCa3.1 channel activation. Cell Calcium. 2016;59:198-207 pubmed publisher
	Yap F, Weber D, Taylor M, Townsley M, Comer B, Maylie J, et al. Endothelial SK3 channel-associated Ca2+ microdomains modulate blood pressure. Am J Physiol Heart Circ Physiol. 2016;310:H1151-63 pubmed publisher
	Ju C, Wang X, Gao C, Zhang S, Ma X, Liu C. Blockade of KCa3.1 Attenuates Left Ventricular Remodeling after Experimental Myocardial Infarction. Cell Physiol Biochem. 2015;36:1305-15 pubmed
	Lin M, Jian M, Taylor M, Cioffi D, Yap F, Liedtke W, et al. Functional coupling of TRPV4, IK, and SK channels contributes to Ca(2+)-dependent endothelial injury in rodent lung. Pulm Circ. 2015;5:279-90 pubmed publisher
	Diaz P, Wood A, Sibley C, Greenwood S. Intermediate conductance Ca2+-activated K+ channels modulate human placental trophoblast syncytialization. PLoS ONE. 2014;9:e90961 pubmed publisher
	Pabbidi M, Mazur O, Fan F, Farley J, Gebremedhin D, Harder D, et al. Enhanced large conductance K+ channel activity contributes to the impaired myogenic response in the cerebral vasculature of Fawn Hooded Hypertensive rats. Am J Physiol Heart Circ Physiol. 2014;306:H989-H1000 pubmed publisher
	Idris Khodja N, Di Marco P, Farhat M, Geny B, Schini Kerth V. Grape-Derived Polyphenols Prevent Doxorubicin-Induced Blunted EDH-Mediated Relaxations in the Rat Mesenteric Artery: Role of ROS and Angiotensin II. Evid Based Complement Alternat Med. 2013;2013:516017 pubmed publisher
	Weisbrod D, Peretz A, Ziskind A, Menaker N, Oz S, Barad L, et al. SK4 Ca2+ activated K+ channel is a critical player in cardiac pacemaker derived from human embryonic stem cells. Proc Natl Acad Sci U S A. 2013;110:E1685-94 pubmed publisher
	Turner K, Sontheimer H. KCa3.1 modulates neuroblast migration along the rostral migratory stream (RMS) in vivo. Cereb Cortex. 2014;24:2388-400 pubmed publisher
	Cuddapah V, Turner K, Seifert S, Sontheimer H. Bradykinin-induced chemotaxis of human gliomas requires the activation of KCa3.1 and ClC-3. J Neurosci. 2013;33:1427-40 pubmed publisher
	Lambertsen K, Gramsbergen J, Sivasaravanaparan M, Ditzel N, Sevelsted Møller L, Oliván Viguera A, et al. Genetic KCa3.1-deficiency produces locomotor hyperactivity and alterations in cerebral monoamine levels. PLoS ONE. 2012;7:e47744 pubmed publisher
	Bagher P, Beleznai T, Kansui Y, Mitchell R, Garland C, Dora K. Low intravascular pressure activates endothelial cell TRPV4 channels, local Ca2+ events, and IKCa channels, reducing arteriolar tone. Proc Natl Acad Sci U S A. 2012;109:18174-9 pubmed publisher
	Idris Khodja N, Chataigneau T, Auger C, Schini Kerth V. Grape-derived polyphenols improve aging-related endothelial dysfunction in rat mesenteric artery: role of oxidative stress and the angiotensin system. PLoS ONE. 2012;7:e32039 pubmed publisher

image

image 1 :

Western blot analysis using Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051), (1:250): - 1. HEK cells transfected with control vector.2. HEK cells transfected with human KCa3.1.

image 2 :

Multiplex staining of KCa3.1 (SK4) and TRPC1 in mouse brain - Immunohistochemical staining of mouse brain sections using Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) and Anti-TRPC1 Antibody (#ACC-010). TRPC1 staining (blue) is detected in neuroblasts and outside neuroblasts as well (in astrocytes). KCa3.1 (red) is detected in neuroblasts. Merged image demonstrates the partial co-localization between the two.Adapted from Turner, K.L. et al. (2014) Cereb. Cortex. 24, 2388. with permission of Oxford University Press.

product information

CAT :

ALM-051

SKU :

ALM-051_100 mcg

Product Name :

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Monoclonal

Accession :

O15554

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Mouse

Blocking Peptide :

Homology :

KCNN4 from human, rat, and mouse samples

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

IgM

Peptide confirmation :

Reconstitution :

25 µl or 100 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4

Product Page - Scientific background :

KCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel. The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Short Description :

A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel

Long Description :

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) is a highly specific monoclonal antibody directed against an epitope of the human channel. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples.

Negative Control :

Positive Control :

Synonyms :

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNN4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC IFC

Clone :

6C1.

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:250

more info or order :

Alomone Labs product webpage