Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody | Alomone Labs ALM-051-B product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody

catalog :

ALM-051-B

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

6C1.

reactivity :

human, mouse, rat

application :

immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Centeio R, Ousingsawat J, Schreiber R, Kunzelmann K. CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A. Int J Mol Sci. 2021;22: pubmed publisher

image

image 1 :

Cell surface detection of KCNN4 in live intact human THP-1 acute monocytic leukemia cell line: Cell surface detection of KCNN4 in live intact human THP-1 acute monocytic leukemia cell line: Black line: Cells + Streptavidin-AlexaFluor-647. Black line: Cells + Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody (#ALM-051-B), (1:10) + Streptavidin-AlexaFluor-647.

image 2 :

Expression of KCNN4 in live LN-CaP cells - Cell surface detection ofKCNN4 in live intact human LNCaP prostate carcinoma cells. A. Cells were stained with MouseAnti-KCNN4 (KCa3.1 SK4) (extracellular)-Biotin Antibody (#ALM-051-B) (1:15) followed by Streptavidin-AlexaFluor-594 (red). B. Cell nuclei were labeled with the cell permeable dye Hoechst 33342 (blue). C. Merge of the two images.

product information

CAT :

ALM-051-B

SKU :

ALM-051-B_50 mcg

Product Name :

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Monoclonal

Accession :

O15554

Applications :

FC IC IF LCI

Reactivity :

Human Rat Mouse

Host :

Mouse

Blocking Peptide :

Homology :

Recognizes KCNN4 from human, rat, and mouse samples

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

IgM

Peptide confirmation :

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular loop

Label :

Biotin

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4

Product Page - Scientific background :

KCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel. The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.

Applications may also work in :

FC IC IF LCI

Supplier :

Alomone Labs

Target :

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Short Description :

A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel Conjugated to Biotin

Long Description :

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) is a highly specific monoclonal antibody directed against an epitope of the human channel. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples. Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody (#ALM-051-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-Biotin Antibody has been tested in direct flow cytometry and immunocytochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

Positive Control :

Synonyms :

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNN4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

6C1.

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ALM-051), and immunohistochemistry, Direct flow cytometry (labeled antibody).

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage