Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs ALM-051-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO Fluor-488 Antibody

catalog :

ALM-051-AG

clonality :

monoclonal

host :

mouse

conjugate :

ATTO 488

clone name :

6C1.

reactivity :

human, mouse, rat

application :

immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Buks R, Dagher T, Rotordam M, Monedero Alonso D, Cochet S, Gautier E, et al. Altered Ca2+ Homeostasis in Red Blood Cells of Polycythemia Vera Patients Following Disturbed Organelle Sorting during Terminal Erythropoiesis. Cells. 2021;11: pubmed publisher
Centeio R, Ousingsawat J, Schreiber R, Kunzelmann K. CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A. Int J Mol Sci. 2021;22: pubmed publisher
Huang R, Wang F, Yang Y, Ma W, Lin Z, Cheng N, et al. Recurrent activations of transient receptor potential vanilloid-1 and vanilloid-4 promote cellular proliferation and migration in esophageal squamous cell carcinoma cells. FEBS Open Bio. 2019;9:206-225 pubmed publisher
Chimote A, Balajthy A, Arnold M, Newton H, Hajdu P, Qualtieri J, et al. A defect in KCa3.1 channel activity limits the ability of CD8+ T cells from cancer patients to infiltrate an adenosine-rich microenvironment. Sci Signal. 2018;11: pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-ZIP8 (SLC39A8) (extracellular) Antibody(#AZT-008) (1:200).2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ALM-051-AG

SKU :

ALM-051-AG_50 mcg

Product Name :

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Monoclonal

Accession :

O15554

Applications :

FC IC IF LCI

Reactivity :

Human Rat Mouse

Host :

Mouse

Blocking Peptide :

Homology :

Recognizes KCNN4 from human, rat, and mouse samples

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

IgM

Peptide confirmation :

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular loop

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4

Product Page - Scientific background :

KCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel. The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.

Applications may also work in :

FC IC IF LCI

Supplier :

Alomone Labs

Target :

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Short Description :

A Mouse Monoclonal Antibody to KCNN4 (KCa3.1, SK4) Channel Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) is a highly specific monoclonal antibody directed against an epitope of the human channel. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples. Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO Fluor-488 Antibody (#ALM-051-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO Fluor-488 Antibody has been tested in live cell imaging applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

Positive Control :

Synonyms :

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNN4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

6C1.

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ALM-051), and immunocytochemistry (labeled antibody).

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage