Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody | Alomone Labs ALM-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody

catalog :

ALM-031

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3C3.

reactivity :

human, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Schilardi G, Kleinlogel S. Two Functional Classes of Rod Bipolar Cells in the Healthy and Degenerated Optogenetically Treated Murine Retina. Front Cell Neurosci. 2021;15:809531 pubmed publisher
Eckrich S, Hecker D, Sorg K, Blum K, Fischer K, M xfc nkner S, et al. Cochlea-Specific Deletion of Cav1.3 Calcium Channels Arrests Inner Hair Cell Differentiation and Unravels Pitfalls of Conditional Mouse Models. Front Cell Neurosci. 2019;13:225 pubmed publisher
Zhang Y, Welzig C, Haburcak M, Wang B, Aronovitz M, Blanton R, et al. Targeted disruption of glycogen synthase kinase-3β in cardiomyocytes attenuates cardiac parasympathetic dysfunction in type 1 diabetic Akita mice. PLoS ONE. 2019;14:e0215213 pubmed publisher
Fell B, Eckrich S, Blum K, Eckrich T, Hecker D, Obermair G, et al. ?2?2 Controls the Function and Trans-Synaptic Coupling of Cav1.3 Channels in Mouse Inner Hair Cells and Is Essential for Normal Hearing. J Neurosci. 2016;36:11024-11036 pubmed
Kammerer S, Jahn S, Winter E, Eidenhammer S, Rezania S, Regitnig P, et al. Critical evaluation of KCNJ3 gene product detection in human breast cancer: mRNA in situ hybridisation is superior to immunohistochemistry. J Clin Pathol. 2016;69:1116-1121 pubmed publisher
Mitrofanova L, Gorshkov A, Lebedev D, Mikhaylov E. Evidence of specialized tissue in human interatrial septum: histological, immunohistochemical and ultrastructural findings. PLoS ONE. 2014;9:e113343 pubmed publisher
Pabbidi M, Mazur O, Fan F, Farley J, Gebremedhin D, Harder D, et al. Enhanced large conductance K+ channel activity contributes to the impaired myogenic response in the cerebral vasculature of Fawn Hooded Hypertensive rats. Am J Physiol Heart Circ Physiol. 2014;306:H989-H1000 pubmed publisher
Rao C, Prodromakis T, Kolker L, Chaudhry U, Trantidou T, Sridhar A, et al. The effect of microgrooved culture substrates on calcium cycling of cardiac myocytes derived from human induced pluripotent stem cells. Biomaterials. 2013;34:2399-411 pubmed publisher

image

image 1 :

Western blot analysis using Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody (#ALM-031), (1:200): - 1. HEK cells transfected with human Kir3.1.2. HEK cells transfected with control vector.3. Mouse heart lysate.

image 2 :

Expression of Kir3.1 in live intact human MCF-7 breast adenocarcinoma cell line - Cell surface detection ofKir3.1 in live intact human MCF-7 breast carcinoma cells. A. Cells were stained with Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody(#ALM-031) (1:20) followed by goat anti-mouse-DyLight-594 secondary antibody (red). B. Live view of the cells. C. Merge of the two images.

image 3 :

Alomone Labs Clobenpropit dihydrobromide inhibits NMDA receptor channels expressed in Xenopus oocytes. - A. Representative time course of NR1/NR2A currents elicited by transient stimulations with 100 M glutamate + 100 M glycine every 50 sec while membrane potential was held at -80 mV and reversibly inhibited by 5 M Clobenpropit dihydrobromide (#C-345). B. Superimposed current traces from the recording shown in A after application of control and 5 M Clobenpropit dihydrobromide as indicated. Transient stimulation is indicated by the arrow.

product information

CAT :

ALM-031

SKU :

ALM-031_100 mcg

Product Name :

Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Monoclonal

Accession :

P48549

Applications :

IC IF LCI WB

Reactivity :

Human Rat

Host :

Mouse

Blocking Peptide :

Homology :

Kir3.1 from human, rat and mouse samples

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

IgG1

Peptide confirmation :

Reconstitution :

25 µl or 100 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

Synthetic peptide mapping to the extracellular loop of human GIRK1

Product Page - Scientific background :

Kir3.1 (or G-protein regulated inward-rectifier K+ channel 1, GIRK1) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir3.1 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Gα-GTP from the Gβγ dimer. The latter directly binds to Kir3 and activates the channel1,3.In the heart, Kir3.1 co-assembles with Kir3.4 to form the prototypical muscarinic-gated K+ channel KAch current, responsible for slowing the heart rate in response of parasympathetic stimulation2.In the brain, Kir3.1 co-assembles with Kir3.2 and mediates the inhibitory effects of many neurotransmitters including opioid, adrenergic, muscarinic, dopaminergic and γ-aminobutyric acid (GABA)1,3.A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.1 containing channels (8.6 nM for the Kir3.1/3.4 combination and 5.4 nM for the Kir3.1/3.2)4,5.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

KCNJ3, G protein-activated inward rectifier potassium channel 1

Short Description :

A Mouse Monoclonal Antibody to GIRK1 (Kir3.1) Channel

Long Description :

Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody (#ALM-031) is a highly specific mouse monoclonal antibody directed against an epitope of the human protein. The antibody can be used in western blot, and immunocytochemistry applications. It has been designed to recognize Kir3.1 from human, rat and mouse samples.

Negative Control :

Positive Control :

Synonyms :

KCNJ3, G protein-activated inward rectifier potassium channel 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

3C3.

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage