Mouse Anti-Human Orai1 (extracellular) Antibody | Alomone Labs ALM-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Mouse Anti-Human Orai1 (extracellular) Antibody

catalog :

ALM-025

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3F11/D10/B9.

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Jairaman A, McQuade A, Granzotto A, Kang Y, Chadarevian J, GANDHI S, et al. TREM2 regulates purinergic receptor-mediated calcium signaling and motility in human iPSC-derived microglia. elife. 2022;11: pubmed publisher
Hoang A, Yu H, R xf6 szer T. Transcriptional Landscaping Identifies a Beige Adipocyte Depot in the Newborn Mouse. Cells. 2021;10: pubmed publisher
Baine S, Bonilla I, Belevych A, Stepanov A, Dorn L, Terentyeva R, et al. Pyridostigmine improves cardiac function and rhythmicity through RyR2 stabilization and inhibition of STIM1-mediated calcium entry in heart failure. J Cell Mol Med. 2021;25:4637-4648 pubmed publisher
Movsisyan N, Pardo L. Kv10.1 Regulates Microtubule Dynamics during Mitosis. Cancers (Basel). 2020;12: pubmed publisher
Bonilla I, Belevych A, Baine S, Stepanov A, Mezache L, Bodnar T, et al. Enhancement of Cardiac Store Operated Calcium Entry (SOCE) within Novel Intercalated Disk Microdomains in Arrhythmic Disease. Sci Rep. 2019;9:10179 pubmed publisher
He X, Song S, Ayon R, Balisterieri A, Black S, Makino A, et al. Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells. Am J Physiol Cell Physiol. 2018;314:C504-C517 pubmed publisher

image

image 1 :

Western blot analysis using Mouse Anti-Human Orai1 (extracellular) Antibody (#ALM-025), (1:200): - 1. HEK-293 cells transfected with human Orai1.2. HEK-293 cells transfected with control vector.

image 2 :

Cell surface detection of Orai1 in intact living Jurkat cells: - ___Unstained cells.___Cells + MouseAnti-Human Orai1 (extracellular)Antibody (#ALM-025) (5-10 g antibody/0.5-1 x 106cells).

image 3 :

Expression of Orai1 in HEK-293 transfected cells - Cell surface detection ofOrai1 in intact living HEK-293 cells expressing Orai1. A. Extracellular staining of HEK-293 cells transfected with Orai1 using MouseAnti-Human Orai1 (extracellular)Antibody (#ALM-025) (red). B. Cells transfected with the empty vector show no Orai1 staining.

product information

CAT :

ALM-025

SKU :

ALM-025_100 mcg

Product Name :

Mouse Anti-Human Orai1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Monoclonal

Accession :

Q96D31

Applications :

IC IF IFC LCI WB

Reactivity :

Human

Host :

Mouse

Blocking Peptide :

Homology :

Orai1 from human samples only

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

IgM

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

25 µl or 100 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

Synthetic peptide mapping to an extracellular sequence of human Orai1

Product Page - Scientific background :

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Short Description :

A Mouse Monoclonal Antibody to Human Orai1 Channel

Long Description :

Mouse Anti-Human Orai1 (extracellular) Antibody (#ALM-025) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only.

Negative Control :

Positive Control :

Synonyms :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ORAI1

AB Specifictiy :

Recognizes only human Orai1.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

3F11/D10/B9.

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage