antibody

Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody

ALM-025-FR

monoclonal

mouse

nonconjugated

3F11/D10/B9.

ALM-025-FR_50 mcg

Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody

Antibodies

Antibodies

Monoclonal

Q63633

FC LCI

Human

Mouse

NA

Recognizes Orai1 from human samples only

PBS pH7.4, 1% BSA with 0.05% sodium azide

IgM

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular

ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

Synthetic peptide mapping to an extracellular sequence of human Orai1

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

FC LCI

Alomone Labs

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye ATTO-633

Mouse Anti-Human Orai1 (extracellular) Antibody (#ALM-025) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only. Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody (#ALM-025-FR) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

NA

NA

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ORAI1

Recognizes only human Orai1.

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

FC

3F11/D10/B9.

Western blot analysis (unlabeled antibody, #ALM-025), and direct flow cytometry (labeled antibody).

NA

Contact Alomone for information

Contact Alomone for information