Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody | Alomone Labs ALM-025-B product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody

catalog :

ALM-025-B

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

3F11/D10/B9.

reactivity :

human

application :

western blot, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Gawali V, Chimote A, Newton H, Feria Garz xf3 n M, Chirra M, Janssen E, et al. Immune Checkpoint Inhibitors Regulate K+ Channel Activity in Cytotoxic T Lymphocytes of Head and Neck Cancer Patients. Front Pharmacol. 2021;12:742862 pubmed publisher
Movsisyan N, Pardo L. Kv10.1 Regulates Microtubule Dynamics during Mitosis. Cancers (Basel). 2020;12: pubmed publisher

image

image 1 :

Western blot analysis using Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody (#ALM-025-B), (1:500): - 1. Jurkat cells.2. HEK cells transfected with human Orai1.3. HEK cells transfected with control vector.

image 2 :

Cell surface detection of Orai1 in intact living Jurkat cells: Cell surface detection of Orai1 in intact living Jurkat cells: Black line: Unstained cells. Green line: Cells + Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody (#ALM-025-B), (5-10 µg antibody/0.5-1 x 106 cells).

image 3 :

Alomone Labs L-689560 inhibits NMDA receptor channels expressed in Xenopus oocytes. - A. Representative time course of NR1/NR2A currents elicited by transient stimulations with 100 M glutamate + 100 M glycine every 50 sec while membrane potential was held at -60 mV. 4 min applications of 5 nM and 10 nM L-689560 (#L-225) reversibly inhibit current amplitude. B. Superimposed current traces from the recording shown in A following application of control 5 nM and 10 nM L-689560 (as indicated). Transient stimulation is indicated by the top horizontal bar.

product information

CAT :

ALM-025-B

SKU :

ALM-025-B_50 mcg

Product Name :

Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Monoclonal

Accession :

Q63633

Applications :

FC WB

Reactivity :

Human

Host :

Mouse

Blocking Peptide :

Homology :

Recognizes Orai1 from human samples only

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

IgM

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular

Label :

Biotin

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

Synthetic peptide mapping to an extracellular sequence of human Orai1

Product Page - Scientific background :

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

Applications may also work in :

FC WB

Supplier :

Alomone Labs

Target :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Short Description :

A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to Biotin

Long Description :

Mouse Anti-Human Orai1 (extracellular) Antibody (#ALM-025) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only. Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody (#ALM-025-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Mouse Anti-Human Orai1 (extracellular)-Biotin Antibody has been tested in western blot and direct flow cytometry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

Positive Control :

Synonyms :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ORAI1

AB Specifictiy :

Recognizes only human Orai1.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

3F11/D10/B9.

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ALM-025), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage