Mouse NGF/proNGF Neutralizing Antibody | Alomone Labs ALM-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Mouse NGF/proNGF Neutralizing Antibody

catalog :

ALM-006

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HAL06.

reactivity :

human, mouse, rat

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Hu J, Gao Y, Huang Q, Wang Y, Mo X, Wang P, et al. Flotillin-1 Interacts With and Sustains the Surface Levels of TRPV2 Channel. Front Cell Dev Biol. 2021;9:634160 pubmed publisher
Griffin N, Rowe C, Gao F, Jobling P, Wills V, Walker M, et al. Clinicopathological Significance of Nerves in Esophageal Cancer. Am J Pathol. 2020;190:1921-1930 pubmed publisher
Adi T, Estacion M, Schulman B, Vernino S, Dib Hajj S, Waxman S. A novel gain-of-function Nav1.7 mutation in a carbamazepine-responsive patient with adult-onset painful peripheral neuropathy. Mol Pain. 2018;14:1744806918815007 pubmed publisher
Oguro A, Inoue T, Kudoh S, Imaoka S. 14,15-epoxyeicosatrienoic acid produced by cytochrome P450s enhances neurite outgrowth of PC12 and rat hippocampal neuronal cells. Pharmacol Res Perspect. 2018;6:e00428 pubmed publisher
Kisiswa L, Fernández Suárez D, Sergaki M, Ibanez C. RIP2 Gates TRAF6 Interaction with Death Receptor p75NTR to Regulate Cerebellar Granule Neuron Survival. Cell Rep. 2018;24:1013-1024 pubmed publisher
Turnquist C, Horikawa I, Foran E, Major E, Vojtesek B, Lane D, et al. p53 isoforms regulate astrocyte-mediated neuroprotection and neurodegeneration. Cell Death Differ. 2016;23:1515-28 pubmed publisher

image

image 1 :

Indirect ELISA of adsorbed proNGF and NGF - Increasing amounts ofRecombinant mouse proNGF protein(#N-250) (blue) andNative mouse NGF 2.5S protein (99%) (#N-240) (red) were adsorbed to a microplate and detected with MouseNGF/proNGF Neutralizing Antibody(#ALM-006) (2 ?g/ml) followed by goat-anti-mouse-HRP and chromogenic substrate.

image 2 :

Indirect ELISA of adsorbed neurotrophic factors - Neurotrophic factorsNative mouse NGF 2.5S protein (99%)(#N-240)Native mouse NGF 7S protein(#N-130)Recombinant human beta-NGF protein(#N-245)Recombinant human proNGF protein(#N-280)Recombinant human BDNF protein(#B-250)Recombinant human Neurotrophin-3 (NT-3) protein(#N-260) andRecombinant human Neurotrophin-4 (NT-4) protein(#N-270) all at 2 ?g/ml were adsorbed to a microplate and analyzed using Mouse NGF/proNGF Neutralizing Antibody(#ALM-006) (2 ?g/ml) followed by goat-anti-mouse-HRP and chromogenic substrate.

image 3 :

Neutralization of NGF-mediated PC12 proliferation - PC12 cells were grown in the absence of serum and in the presence ofNative mouse NGF 2.5S protein (99%)(#N-240) (50 ng/ml) as indicated. Varying concentrations of MouseNGF/proNGF Neutralizing Antibody(#ALM-006) were added to the culture and cell viability was established after 24 h using the XTT method. Note that 600 ng/ml Mouse NGF/proNGF Neutralizing Antibody was able to completely block the activity of 50 ng/ml mouse NGF.

product information

CAT :

ALM-006

SKU :

ALM-006_0.25 mg

Product Name :

Mouse NGF/proNGF Neutralizing Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Monoclonal

Accession :

P01139

Applications :

IE N

Reactivity :

Human Rat

Host :

Mouse

Blocking Peptide :

Homology :

NGF and proNGF from human, rat and mouse samples

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

IgG1

Peptide confirmation :

Reconstitution :

50 µl or 0.25 ml double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

Highly purified 2.5S mouse NGF

Product Page - Scientific background :

The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.4 NGF is purified in three forms: the 7S, 2.5S and β. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the β form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.7 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.NGF has been shown to regulate neuronal survival, development function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11 The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12

Applications may also work in :

IE N

Supplier :

Alomone Labs

Target :

Nerve growth factor

Short Description :

A Mouse Monoclonal Antibody to NGF and proNGF Designed to Neutralize their Biological Functions

Long Description :

Alomone Labs is pleased to offer a highly specific monoclonal antibody directed against an epitope of mouse NGF. Mouse NGF/proNGF Neutralizing Antibody (#ALM-006) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF. It was designed to recognize NGF and proNGF from human, rat and mouse samples.

Negative Control :

Positive Control :

Synonyms :

Nerve growth factor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NGF

AB Specifictiy :

The antibody has minimal cross-reactivity with BDNF, NT-3, and NT-4/5.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

HAL06.

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

Application Dilutions: Western blot wb :

more info or order :

Alomone Labs product webpage