Anti-KCC2 (SLC12A5) Antibody | Alomone Labs AKT-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCC2 (SLC12A5) Antibody

catalog :

AKT-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:500).2. Anti-KCC2 (SLC12A5) Antibody, preincubated with KCC2/SLC12A5 Blocking Peptide (#BLP-KT005).

image 2 :

Expression of KCC2 in rat cerebellum and hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. KCC2 immunoreactivity in the cerebellum (green) is detected in the granule layer (GL) and molecular layer (MOL). B. KCC2 staining in the hippocampal dentate gyrus region (green) is observed in the outer molecular layer (OML) and the hilus (H). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AKT-005

SKU :

AKT-005-CF_0.2 ml

Product Name :

Anti-KCC2 (SLC12A5) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63633

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-KT005

Homology :

Human - identical; mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KNPANTRLRLNVPE, corresponding to amino acid residues 967-980 of rat KCC2

Product Page - Scientific background :

KCC2 (K+-Cl−-cotransporter 2) is a glycoprotein neuron-specific cotransporter and a member of the cation-chloride co-transporter (CCC) family encoded by the Slc12a5 gene. KCC2 is responsible for maintaining low Cl− levels in neurons using the potassium gradient. In addition, KCC2 regulates the formation, functional maintenance and plasticity of glutamatergic synapses. The proper inhibitory functions of GABA are maintained by KCC2 since GABA is dependent on the Cl− driving force promoted by the intracellular concentration of Cl− 1,2.KCC2 has a predicted structure of 12 transmembrane segments, six extracellular loops flanked by a short intracellular amino terminal and a long intracellular carboxy-terminal1,2.KCC2 protein expression is detected in the central nervous system including the spinal cord, thalamus, cerebellum, hippocampus, cortical structures and the auditory brainstem1.Human KCC2 mutations are associated with severe early-onset epileptic encephalopathy and febrile seizures1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

K+-Cl- cotransporter 2, Electroneutral potassium-chloride cotransporter 2, Neuronal K-Cl cotransporter, Solute carrier family 12 member 5

Short Description :

A Rabbit Polyclonal Antibody to KCC2 Transporter

Long Description :

Anti-KCC2 (SLC12A5) Antibody (#AKT-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCC2 from human, rat, and mouse samples.

Negative Control :

BLP-KT005

Positive Control :

Synonyms :

K+-Cl- cotransporter 2, Electroneutral potassium-chloride cotransporter 2, Neuronal K-Cl cotransporter, Solute carrier family 12 member 5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC12A5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage