Anti-KCC1 (SLC12A4) Antibody | Alomone Labs AKT-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCC1 (SLC12A4) Antibody

catalog :

AKT-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Tang L, Fang X, Winesett S, Cheng C, Binder H, Rivkees S, et al. Bumetanide increases Cl--dependent short-circuit current in late distal colon: Evidence for the presence of active electrogenic Cl- absorption. PLoS ONE. 2017;12:e0171045 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human K562 erythroleukemia cell lysates (lanes 3 and 6): - 1-3. Anti-KCC1 (SLC12A4) Antibody (#AKT-001), (1:200).4-6. Anti-KCC1 (SLC12A4) Antibody, preincubated with KCC1/SLC12A4 Blocking Peptide (#BLP-KT001).

image 2 :

Peptide (C)HAPDNFRELVHIK corresponding to amino acid residues 998-1010 of rat KCC1 (AccessionQ63632). Intracellular C-terminus.

image 3 :

Western blot analysis of rat lung (lanes 1 and 4 1:200) mouse heart (lanes 2 and 5 1:200) and rat aortic endothelial cell (RAEC) (lanes 3 and 6 1:400) lysates: - 1-3. Anti-RAMP2 (extracellular)Antibody (#ARR-022).4-6. Anti-RAMP2 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AKT-001

SKU :

AKT-001-CF_0.2 ml

Product Name :

Anti-KCC1 (SLC12A4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63632

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-KT001

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HAPDNFRELVHIK, corresponding to amino acid residues 998-1010 of rat KCC1

Product Page - Scientific background :

Potassium-chloride cotransporters (KCCs) are members of the cation chloride cotransporter gene (Slc) superfamily. This family consists of sodium-potassium-two chloride cotransporter 1 (NKCC1) and 2 (NKCC2), sodium-chloride cotransporter (NCC) and KCCs1. All of these transporters mediate electroneutral transport of cations coupled with one or two Cl- ions and contribute to regulation of cell volume and homeostasis of intracellular Cl- concentration. KCCs extrude one K+ with one Cl- from the intracellular space to the extracellular space by using the chemical gradient of K+ as the driving force2.Four KCC isoforms (KCC1, KCC2, KCC3 and KCC4) have been identified in mammalian species3.K-Cl cotransporters regulate neuronal and glial electrochemical equilibrium potential for Cl-, and so can determine the excitatory or inhibitory influences of GABA- and glycine-gated Cl- channels4. KCC1 is a member of the cation-chloride cotransporter (CCC) superfamily of proteins, as with all CCC proteins, KCC1 is an integral membrane protein with 12 transmembrane domains and both N- and C-terminal cytoplasmic domains 5. KCC1 is ubiquitously expressed6.K-Cl cotransport activity is elevated in red blood cells of individuals with sickle cell disease, thalassemia, and diseases associated with other mutant Hb. Human erythrocytes from individuals with the reticulocytotic anemic states of sickle cell disease, Hb SC disease, and β-thalassemia intermedia exhibit uniformly elevated KCC1-like immunoreactivity compared with normal AA erythrocytes7.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Erythroid K+-Cl- cotransporter 1, Electroneutral potassium-chloride cotransporter 1

Short Description :

A Rabbit Polyclonal Antibody to KCC1 Transporter

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against epitope of rat KCC1. Anti-KCC1 (SLC12A4) Antibody (#AKT-001) can be used in western blot analysis. It has been designed to recognize KCC1 from rat, mouse and human samples. Several splice variants of the KCC1 human gene have been identified. This antibody will recognize all known variants except for isoform 4. For more information go to human KCC1 (Accession Q9UP95).

Negative Control :

BLP-KT001

Positive Control :

Synonyms :

Erythroid K+-Cl- cotransporter 1, Electroneutral potassium-chloride cotransporter 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC12A4

AB Specifictiy :

Several splice variants of the KCC1 human gene have been identified. This antibody will recognize all known variants except for isoform 4. For more information go to human KCC1 (Accession Q9UP95).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage