Anti-KISS1 Receptor (GPR54) (extracellular) Antibody | Alomone Labs AKR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KISS1 Receptor (GPR54) (extracellular) Antibody

catalog :

AKR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Gao Z, Ma X, Liu J, Ge Y, Wang L, Fu P, et al. Troxerutin protects against DHT-induced polycystic ovary syndrome in rats. J Ovarian Res. 2020;13:106 pubmed publisher
Cielesh M, McGrath B, Scott C, Norman S, Stephen C. The localization of kisspeptin and kisspeptin receptor in the canine ovary during different stages of the reproductive cycle. Reprod Domest Anim. 2017;52 Suppl 2:24-28 pubmed publisher
Dotterweich J, Tower R, Brandl A, Muller M, Hofbauer L, Beilhack A, et al. The KISS1 Receptor as an In Vivo Microenvironment Imaging Biomarker of Multiple Myeloma Bone Disease. PLoS ONE. 2016;11:e0155087 pubmed publisher

image

image 1 :

Western blot analysis of Jurkat (lanes 1 and 4), HL-60 (lanes 2 and 5), MCF-7 (lanes 3 and 6) and rat brain (lanes 7 and 8) lysates: - 1,2,3,7. Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001), (1:500).4,5,6,8. Anti-KISS1 Receptor (GPR54) (extracellular) Antibody, preincubated with KISS1 Receptor/GPR54 (extracellular) Blocking Peptide (#BLP-KR001).

image 2 :

Expression of KISS1R in rat pancreas - Immunohistochemical staining of paraffin embedded section of rat pancreas using Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001), (1:100). KISS1R staining (brown) appears in Isles of Langerhans (IL). Hematoxilin is used as the counterstain.

image 3 :

Expression of KISS1R in rat pancreas - Immunohistochemical staining of paraffin embedded section of rat pancreas using Anti-KISS1 Receptor (GPR54) (extracellular) Antibody(#AKR-001) (1:100). KISS1R staining (brown) appears in Isles of Langerhans (IL). Hematoxilin is used as the counterstain.

product information

CAT :

AKR-001

SKU :

AKR-001-CF_0.2 ml

Product Name :

Anti-KISS1 Receptor (GPR54) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q969F8

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-KR001

Homology :

Rat - 13/14 amino acid residues identical; mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)GSWHPRSYAAYALK, corresponding to amino acid residues 292-305 of human KISS1R

Product Page - Scientific background :

The kisspeptin receptor (KISS1R, GPCR54) is a member of the G-protein coupled receptor superfamily. KISS1R natural ligands are peptides called kisspeptins which are encoded by the Kiss1 gene. The Kiss1 gene was originally discovered by Hershey scientists who named it after their famous chocolate, the "Kiss".The kisspeptins, acting through KISS1R, were found to be potent inhibitors of metastasis in a variety of cancer cell types including thyroid and pancreatic cancer cells. KISS1R and kisspeptins are involved in the direct regulation of the reproductive neuroendocrine axis.KISS1R is widely distributed throughout the brain, where it is most highly expressed in the hypothalamic and amygdaloid nuclei. High expression was also found in the pancreas and placenta. Lower-level expression was found in a variety of other peripheral tissues including peripheral blood leukocytes, kidney, lung, testes, spleen, thymus, adrenal glands and lymph nodes.1-3

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12

Short Description :

A Rabbit Polyclonal Antibody to Kisspeptin Receptor

Long Description :

Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001) is a highly specific antibody directed against an epitope of the human kisspeptin receptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KISS1R from human, rat, and mouse samples.

Negative Control :

BLP-KR001

Positive Control :

Synonyms :

Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KISS1R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage