Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody | Alomone Labs AKR-001-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody

catalog :

AKR-001-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of KISS1R by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:

image 2 :

Cell surface detection of KISS1R by direct flow cytometry in live intact mouse J774 macrophage cell line:

image 3 :

Cell surface detection of KISS1R by direct flow cytometry in live intact human Jurkat T-cell leukemia cell line:

product information

CAT :

AKR-001-F

SKU :

AKR-001-F_50 mcl

Product Name :

Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q969F8

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-KR001

Homology :

Rat - 13 out of 14 amino acid residues identical, Mouse - 12 out of 14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)GSWHPRSYAAYALK, corresponding to amino acid residues 292-305 of human KISS1R

Product Page - Scientific background :

The kisspeptin receptor (KISS1R, GPCR54) is a member of the G-protein coupled receptor superfamily. KISS1R natural ligands are peptides called kisspeptins which are encoded by the Kiss1 gene. The Kiss1 gene was originally discovered by Hershey scientists who named it after their famous chocolate, the "Kiss".The kisspeptins, acting through KISS1R, were found to be potent inhibitors of metastasis in a variety of cancer cell types including thyroid and pancreatic cancer cells. KISS1R and kisspeptins are involved in the direct regulation of the reproductive neuroendocrine axis.KISS1R is widely distributed throughout the brain, where it is most highly expressed in the hypothalamic and amygdaloid nuclei. High expression was also found in the pancreas and placenta. Lower-level expression was found in a variety of other peripheral tissues including peripheral blood leukocytes, kidney, lung, testes, spleen, thymus, adrenal glands and lymph nodes.1-3

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12, G-Protein Coupled Receptor 54

Short Description :

A Rabbit Polyclonal Antibody to KISS1 Receptor (GPR54) (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001) is a highly specific antibody directed against an epitope of human KISS1 receptor. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize KISS1R from human, rat, and mouse samples. Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody (#AKR-001-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12, G-Protein Coupled Receptor 54

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KISS1R

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AKR-001), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage