Anti-Ferroportin (SLC40A1) Antibody | Alomone Labs AIT-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Ferroportin (SLC40A1) Antibody

catalog :

AIT-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Chiou B, Neal E, Bowman A, Lippmann E, Simpson I, Connor J. Endothelial cells are critical regulators of iron transport in a model of the human blood-brain barrier. J Cereb Blood Flow Metab. 2019;39:2117-2131 pubmed publisher

image

image 1 :

Western blot analysis of rat spleen (lanes 1 and 5), rat small intestine (lanes 2 and 6), rat liver (lanes 3 and 7) and mouse liver (lanes 4 and 8) lysates. - 1-4. Anti-Ferroportin (SLC40A1) Antibody (#AIT-001), (1:500).5-8. Anti-Ferroportin (SLC40A1) Antibody, preincubated with Ferroportin/SLC40A1 Blocking Peptide (#BLP-IT001).

image 2 :

Expression of Ferroportin in mouse brain - Immunohistochemical staining of mouse cerebellum and forebrain using Anti-Ferroportin (SLC40A1) Antibody (#AIT-001). A. In the cerebellum, Ferroportin staining (green) appears in cerebellar purkinje cells (horizontal arrows) and their dendrites (vertical arrows). B. In the forebrain, intense Ferroportin staining appears (green) in oligodendrocytes (horizontal arrows) and moderate staining is detected in neurons (vertical arrow). In both panels (A and B) DAPI is used as the counterstain.

image 3 :

Expression of Ferroportin in mouse brain - Immunohistochemical staining of mouse cerebellum and forebrain usingAnti-Ferroportin (SLC40A1) Antibody(#AIT-001). A. In the cerebellum Ferroportin staining (green) appears in cerebellar purkinje cells (horizontal arrows) and their dendrites (vertical arrows). B. In the forebrain intense Ferroportin staining appears (green) in oligodendrocytes (horizontal arrows) and moderate staining is detected in neurons (vertical arrow). In both panels (A and B) DAPI is used as the counterstain.

product information

CAT :

AIT-001

SKU :

AIT-001-CF_0.2 ml

Product Name :

Anti-Ferroportin (SLC40A1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9NP59

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IT001

Homology :

Mouse, rat - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop Note: the structure of Ferroportin is still controversial and there is no defined topology yet.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EGTHLMGVKDSNIHE, corresponding to amino acid residues 261-275 of human Ferroportin

Product Page - Scientific background :

Ferroportin (SLC40A1) is an iron transporter suggested to play roles in intestinal iron absorption and cellular iron release. To date, Ferroportin is the only known mammalian iron exporter and is essential for transport of iron from one cell type to another1.Ferroportin has 9-12 transmembrane domains. The topology of the transporter has not been defined with precision and the number of transmembrane domains is still under scrutiny. Studies indicate that the amino terminus of Ferroportin is cytosolic. The location of the carboxyl-terminal, however, is unclear. A second controversial issue regarding Ferroportin structure is whether Ferroportin is a monomer or dimer. Many reports using similar approaches including chromatography, cross-linking and physical techniques such as birefringence, have led to contrasting results2.Ferroportin is found in all the tissues where major iron flows are regulated, including duodenal enterocytes, placental trophoblast, macrophages, and hepatocytes3.Mutations that result in defective Ferroportin transport activity will lead to the phenotype of "classic" Ferroportin disease, which is macrophage iron loading and normal to low transferrin saturation2. Expression of mutant Ferroportin in some cultured cell types results in defects in Ferroportin trafficking where the mutant protein never reaches the cell surface4. In other cell types, the mutant Ferroportin was shown to accumulate at the cell surface but had defective transport activity5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Ferroportin-1, FPN1, Iron-regulated transporter 1, IREG1, Metal transporter protein, MTP1, SLC11A3, HFE4

Short Description :

A Rabbit Polyclonal Antibody to Ferroportin (SLC40A1)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Ferroportin-1. Anti-Ferroportin (SLC40A1) Antibody (#AIT-001) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Ferroportin-1 from human, rat, and mouse samples.

Negative Control :

BLP-IT001

Positive Control :

Synonyms :

Ferroportin-1, FPN1, Iron-regulated transporter 1, IREG1, Metal transporter protein, MTP1, SLC11A3, HFE4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC40A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage