Anti-Septin-7 Antibody | Alomone Labs AIP-047 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Septin-7 Antibody

catalog :

AIP-047

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat spleen (lanes 1 and 4), mouse spleen (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates: - 1-3. Anti-Septin-7 Antibody (#AIP-047), (1:200). 4-6. Anti-Septin-7 Antibody, preincubated with Septin-7 Blocking Peptide (#BLP-IP047) (#BLP-IP047).

product information

CAT :

AIP-047

SKU :

AIP-047-CF_0.2 ml

Product Name :

Anti-Septin-7 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WVC0

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP047

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

Peptide (C)HRIKKTVQVEQSK, corresponding to amino acid residues 83-96 of rat Septin-7

Product Page - Scientific background :

Septin 7 is a highly conserved GTP-binding filamentous protein. Septins are important for the organization of the actin cytoskeleton. Septin7 also plays an important role in cytokinesis, and membrane dynamics1,2.Septins were first discovered in yeast and since, 13 septin genes have been identified in mammals (SEPT1 to SEPT12 and SEPT14). The 13 gene-encoded proteins can be classified into four distinct subgroups based on their sequence homology of their domain structure2.Septins share a highly conserved structure composed of the central GTPase domain and N- and C-terminal domains of various lengths3. They self-assemble into higher-order structures, including filaments and rings in tri-, hexa-, or octameric complexes consisting of multiple septin polypeptides, which are typical for different cell types. The various septin isoforms seem to determine the function and regulation of the septin complex4.Septin-7 regulates Ca2+ entry through Orai1 by regulating the opening of the channel5.Dysregulation of septins leads to human diseases such as neurodegenerative and bleeding disorders. In non-dividing cells such as neuronal tissue and platelets septins have been associated with exocytosis1,2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Sept7, CDC10, SEPT7A, NBLA02942, CDC3

Short Description :

A Rabbit Polyclonal Antibody to Septin-7

Long Description :

Anti-Septin-7 Antibody (#AIP-047) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Septin-7 from human, rat, and mouse samples.

Negative Control :

BLP-IP047

Positive Control :

Synonyms :

Sept7, CDC10, SEPT7A, NBLA02942, CDC3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SEPTIN7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage