Anti-CRMP2 Antibody | Alomone Labs AIP-029 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CRMP2 Antibody

catalog :

AIP-029

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human CCF-STTGI brain astrocytoma (lanes 3 and 7) and rat PC-12 peochromocytoma (lanes 4 and 8) cell line lysates: - 1-3. Anti-CRMP2 Antibody (#AIP-029), (1:200). 4. Anti-CRMP2 Antibody (1:500).5-8. Anti-CRMP2 Antibody, preincubated with CRMP2 Blocking Peptide (#BLP-IP029).

image 2 :

Expression of CRMP2 in rat spinal cord. - Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections sections with Anti-CRMP2 Antibody (#AIP-029), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CRMP2 immunoreactivity (green) appears in the superficial layers of the dorsal horn (arrows). B. Pre-incubation of the antibody with CRMP2 Blocking Peptide (BLP-IP029), suppressed staining. Cell nuclei are stained with DAPI (blue)

product information

CAT :

AIP-029

SKU :

AIP-029-CF_0.2 ml

Product Name :

Anti-CRMP2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P47942

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP029

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CEVSVTPKTVTPAS, corresponding to amino acid residues 504-517 of rat CRMP2

Product Page - Scientific background :

Collapsin response mediator protein 2 (CRMP-2, DRP-2), is a kinesin-binding protein and a member of the CRMP family, a novel family of microtubule-associated proteins (MAPs). This family consists of 5 highly conserved phosphorylated proteins: CRMP1-CRMP5. CRMP-2 was originally identified in the regulation of microtubule dimerization. CRMP-2 is the first member of the CRMP family identified in primary neuronal cells1,2.Physiological functions of CRMP-2 protein include regulation of cell surface receptor endocytosis, kinesin-dependent axonal transport, growth cone collapse, polarity and differentiation of developing neurons and neurite outgrowth and the regulation of microtubule dynamics2.Studies suggest that CRMP-2 can modulate neuronal viability through the PI3K/mTOR/S6K pathway. In addition, CRMP-2 expression has been detected in non-neuronal cells such as leukocytes, fibroblasts and neuroblastoma1.Phosphorylation of CRMP-2 on specific Thr and Ser residues inactivates its activity, leading to inhibition of axonal growth and neuronal polarity. Knockdown of CRMP-2 shortens the total dendritic length and decreases dendritic branching3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

CRMP-2, Collapsin response mediator protein 2, Dihydropyrimidinase-related protein 2, DHPRP2, DRP-2, Dpysl2, Turned on after division 64 kDa protein, TOAD-64, Ulip2, Unc-33-like phosphoprotein 2

Short Description :

A Rabbit Polyclonal Antibody to CRMP-2

Long Description :

Anti-CRMP2 Antibody (#AIP-029) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize CRMP-2 from rat, mouse, and human samples.

Negative Control :

BLP-IP029

Positive Control :

Synonyms :

CRMP-2, Collapsin response mediator protein 2, Dihydropyrimidinase-related protein 2, DHPRP2, DRP-2, Dpysl2, Turned on after division 64 kDa protein, TOAD-64, Ulip2, Unc-33-like phosphoprotein 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DPYSL2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage