Anti-CRMP1 Antibody | Alomone Labs AIP-028 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CRMP1 Antibody

catalog :

AIP-028

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 5), rat cortex (lanes 2 and 6), rat new born brain (lanes 3 and 7) and human brain glioblastoma (U-87 MG) cell line lysates: - 1-4. Anti-CRMP1 Antibody (#AIP-028), (1:200).5-8. Anti-CRMP1 Antibody, preincubated with CRMP1 Blocking Peptide (#BLP-IP028).

product information

CAT :

AIP-028

SKU :

AIP-028-CF_0.2 ml

Product Name :

Anti-CRMP1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62950

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP028

Homology :

Mouse - identical; human - 12/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RVRIRSKVFGLHSVSR, corresponding to amino acid residues 481-496 of rat CRMP1

Product Page - Scientific background :

Collapsin response mediator proteins (CRMPs) are a family of cytosolic phosphoproteins that were originally identified in developing nervous tissue. They are characterized as intracellular mediators of Semaphorin 3A signaling pathway, which is involved in axon differentiation during neural development.The CRMP family includes five members; CRMP-1 to CRMP-5. All five isoforms share a significant similarity in sequence with liver dihydropyrimidinase (DHPase) and with each other. CRMP-1 to CRMP-4 share 75% identical sequence, while CRMP-5 shares 50% identity with the other CRMPs.CRMP-1 is involved in cortical neuronal migration through reelin signaling. CRMP-1 inhibits the migration and invasion of non-small cell lung cancer (NSCLC). CRMP-1 includes a long form isoform, LCRMP-1, which promotes filopodia formation, cancer cell migration and invasion, resulting in poor clinical outcomes in NSCLC patients, therefore the long form is considered to be an invasion enhancer in NSCLC1-3.Human CRMP-1 overall structure is very similar to mouse CRMP-1. The human protein generally comprises a heterotetrameric assembly with a randomly coiled link that connects the protomers to each other. The proteins can form homotetramers when purified from bovine brain. CRMPs contains a positively charged C-terminal region that is highly susceptible to proteolysis2,3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

CRMP-1, Collapsin response mediator protein 1, Dihydropyrimidinase-related protein 1, DRP-1, Dpysl1, Dihydropyrimidinase-like 1, Ulip3, Unc-33-like phosphoprotein 3

Short Description :

A Rabbit Polyclonal Antibody to CRMP-1

Long Description :

Anti-CRMP1 Antibody (#AIP-028) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize CRMP-1 from human, mouse, and rat samples.

Negative Control :

BLP-IP028

Positive Control :

Synonyms :

CRMP-1, Collapsin response mediator protein 1, Dihydropyrimidinase-related protein 1, DRP-1, Dpysl1, Dihydropyrimidinase-like 1, Ulip3, Unc-33-like phosphoprotein 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CRMP1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage