Anti-Neurofascin Antibody | Alomone Labs AIP-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neurofascin Antibody

catalog :

AIP-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat hippocampus (lanes 3 and 6) lysates: - 1-3. Anti-Neurofascin Antibody (#AIP-025), (1:800).4-6. Anti-Neurofascin Antibody, preincubated with Neurofascin Blocking Peptide (#BLP-IP025).

image 2 :

Expression of Neurofascin in rat spinal cord. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neurofascin Antibody (#AIP-025), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Neurofascin immunoreactivity (green) appears dense in the dorsal horn (DH) and sparse in the dorsal column (DC). B. Pre-incubation of the antibody with Neurofascin Blocking Peptide (BLP-IP025), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AIP-025

SKU :

AIP-025-CF_0.2 ml

Product Name :

Anti-Neurofascin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97685

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP025

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)REKKDVPLGPEDPK, corresponding to amino acid residues 1142-1155 of rat NFASC

Product Page - Scientific background :

Neurofascin belongs to the L1 subgroup of the immunoglobulin superfamily of cell adhesion molecules and is an axon associated surface glycoprotein implicated in axonal growth and fasciculation.Neurofascin is comprised of four structural elements: At the NH2 terminus, neurofascin contains six Ig-like motifs of the C2 subcategory followed by four fibronectin type III (FNIII)-related repeats. Between the FNIII-like repeats and the plasma membrane spanning region, neurofascin contains a 75-amino acid residues rich in proline, alanine and threonine which might be the target of extensive O-linked glycosylation. A transmembrane segment is followed by a 113-amino acid residues-long cytoplasmic domain1.Neurofascin associates with sodium channel β1 (NaVβ1) units via interaction of its first Ig-like domain and second fibronectin type III-like domain with the extracellular Ig-like domain of β1. NaVβ1 subunits localize to nodes of Ranvier with neurofascin in sciatic nerve axons. NaVβ1 and neurofascin are associated as early as postnatal day 5, during the period that nodes of Ranvier are in formation2.Unlike L1 and other subgroup members, neurofascin is subjected to extensive alternative splicing that is regulated during embryonic development of the brain. An oligodendroglial isoform of neurofascin, NF155, serves as a glial receptor for the paranodin/Caspr-contactin complex. The extracellular domain of NF155 binds to the complex at the cell surface3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

NFASC, NRCAML, NF

Short Description :

A Rabbit Polyclonal Antibody to Neurofascin

Long Description :

Anti-Neurofascin Antibody (#AIP-025) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize NFASC from human, mouse, and rat samples.

Negative Control :

BLP-IP025

Positive Control :

Synonyms :

NFASC, NRCAML, NF

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NFASC

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage