Anti-PACSIN1 Antibody | Alomone Labs AIP-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PACSIN1 Antibody

catalog :

AIP-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3), mouse brain (lanes 2 and 4), (1:200) and human brain neuroblastoma SH-SY5Y cell line lysates (lanes 5 and 6), (1:600): - 1, 2, 5. Anti-PACSIN1 Antibody (#AIP-023). 3, 4, 6. Anti-PACSIN1 Antibody, preincubated with PACSIN1 Blocking Peptide (#BLP-IP023).

image 2 :

Expression of PACSIN1 in rat hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PACSIN1 Antibody (#AIP-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region, showed PACSIN1 immunoreactivity (green) in some neurons (arrows) in the pyramidal layer (P). B. Pre-incubation of the antibody with PACSIN1 Blocking Peptide (BLP-IP023), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AIP-023

SKU :

AIP-023-CF_0.2 ml

Product Name :

Anti-PACSIN1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z0W5

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP023

Homology :

Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EWNPDLPHTAAKKEK corresponding to amino acid residues 301-315 of rat PACSIN1

Product Page - Scientific background :

The protein kinase C and casein kinase substrate in neurons (PACSIN), also known as syndapin, is an F-BAR (elongated BAR) and SH3 (src homology-3) domain-containing protein that is capable of remodeling the plasma membrane and mediating protein-protein interactions.Three different PACSIN variants are identified, which are differentially expressed: PACSIN I is the neuronal-specific isoform, PACSIN II is ubiquitously expressed and PACSIN III is expressed mainly in skeletal muscles and heart1.PACSINs interact with the large GTPase dynamin and several other proteins implicated in vesicle trafficking, PACSIN-dynamin complexes play an important role in vesicle fission at different donor membranes, including the plasma membrane (endocytosis) and Golgi membranes. In addition, PACSINs are implicated in later steps of vesicle cycling in neuronal and non-neuronal cells. PACSINs also engage in additional interactions with molecules involved in several signal transduction pathways, producing crosstalk at the interface between membrane trafficking and the cytoskeleton. Given the distinct expression patterns of the different PACSINs and their splice forms, these proteins could have isoform-specific functions2.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Syndapin-1, Synaptic, Dynamin-associated protein I, Protein kinase C and casein kinase substrate in neurons protein 1

Short Description :

A Rabbit Polyclonal Antibody to PACSIN1

Long Description :

Anti-PACSIN1 Antibody (#AIP-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize PACSIN1 from human, rat, and mouse samples.

Negative Control :

BLP-IP023

Positive Control :

Synonyms :

Syndapin-1, Synaptic, Dynamin-associated protein I, Protein kinase C and casein kinase substrate in neurons protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PACSIN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:600

more info or order :

Alomone Labs product webpage