Anti-CASK Antibody | Alomone Labs AIP-018 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CASK Antibody

catalog :

AIP-018

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell line lysates: - 1,2. Anti-CASK Antibody (#AIP-018), (1:400).3. Anti-CASK Antibody (1:200).4,5,6. Anti-CASK Antibody, preincubated with CASK Blocking Peptide (#BLP-IP018).

image 2 :

Expression of CASK in rat hypothalamus - Immunohistochemical staining of rat hypothalamus free floating frozen sections using Anti-CASK Antibody (#AIP-018), (1:200). CASK staining (green) appears in fibers with varicosities (arrows) running from the ventromedial hypothalamus (VMH) along the base of the brain. Nuclei are stained using DAPI (blue).

product information

CAT :

AIP-018

SKU :

AIP-018-CF_0.2 ml

Product Name :

Anti-CASK Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O70589

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-IP018

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KERDRYAYKIHLPE, corresponding to amino acid residues 277-290 of mouse CASK

Product Page - Scientific background :

The CASK protein belongs to the membrane-associated guanylate kinase family of proteins (MAGUK). MAGUK proteins generally target neuronal synapses and regulate trafficking, targeting, and signaling of ion channels (like CaV channels).CASK consists of the following modular domains- the PDZ, Src homolog 3 (SH3), and guanylate kinase (GK) domains that are the characteristic protein domains shared by all MAGUK proteins. In addition, CASK contains two L27 domains preceding the single PDZ domain and has a unique CaMK-like domain at its N-terminal region.The PDZ domain, consisting of approximately 90 amino acid residues, is specialized for binding to short peptide motifs at the extreme C-terminal tail of its binding partners, although other modes of interaction also occur. The Src homolog 3 (SH3) domain typically interacts with a proline-rich sequence. In addition to interaction with the proline-rich motif, the CASK SH3 domain mediates interaction with the GK domain. This interaction can occur intra- or intermolecularly. CASK protein can therefore form a homodimer via the inter-molecular SH3-GK interaction. Because the CASK SH3 domain also recognizes the GK domain of other MAGUK proteins, such as p55, CASK also forms heterodimers with other MAGUK proteins via an intermolecular SH3-GK interaction. The CASK CaMK-like domain functions as a protein-protein interacting domain, which binds to several cellular proteins, such as Mint1/X11, Caskin 1, CIP98, and Carom.CASK is mostly expressed in (but not restricted to) neurons; expression levels of CASK are 3-5-fold higher in the brain than in other organs. In addition to playing an important role in the nervous system, CASK has also been suggested as having a role in establishing epithelial cell polarity in mammals1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Peripheral plasma membrane protein CASK, Calcium/calmodulin-dependent serine protein kinase, Membrane-associated guanylate kinase, CMG, CAMGUK, LIN2, TNRC8

Short Description :

A Rabbit Polyclonal Antibody to CASK

Long Description :

Anti-CASK Antibody (#AIP-018) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CASK from rat, mouse, and human samples.

Negative Control :

BLP-IP018

Positive Control :

Synonyms :

Peripheral plasma membrane protein CASK, Calcium/calmodulin-dependent serine protein kinase, Membrane-associated guanylate kinase, CMG, CAMGUK, LIN2, TNRC8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CASK

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage